Pub. Date:
Springer-Verlag New York, LLC
DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs / Edition 1

DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs / Edition 1

by Neil Osheroff, Mary-Ann Bjornsti


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Product Details

ISBN-13: 9781617370571
Publisher: Springer-Verlag New York, LLC
Publication date: 11/10/2010
Series: Methods in Molecular Biology , #95
Edition description: Softcover reprint of hardcover 1st ed. 2001
Pages: 334
Product dimensions: 5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Assaying DNA Topoisomerase I Relaxation Activity
Lance Stewart and James J. Champoux

DNA Topoisomerase II-Catalyzed DNA Decatenation
Andrea Haldane and Daniel M. Sullivan

Plasmid DNA Supercoiling by DNA Gyrase
Penny J. Sayer, Martin L. Goble, Mark Oram, and L. Mark Fisher

Analyzing Reverse Gyrase Activity
Michel Duguet, Christine Jaxel, Anne-Céile Déclais, Fabrice Confalonieri, Janine Marsault, Claire Bouthier de la Tour, Marc Nadal, and Christiane Portemer

Topoisomerase II-Catalyzed ATP Hydrolosis as Monitored by Thin-Layer Chromatography
Paul S. Kingma, John M. Fortune, and Neil Osheroff

Use of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II
Janet E. Lindsley

Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift Assay
Stewart Shuman

Filter Binding Assays for Topoisomerase-DNA Complexes
Joaquim Roca

DNA Topoisomerase I-Mediated Nicking of Circular Duplex DNA
James J. Champoux

Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives
Kenneth N. Kreuzer, Catherine H. Freudenreich, and Yves Pommier

Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation
Anni H. Anderson, Kent Christiansen, and Ole Westergaard

Synthesis and Use of DNA Containing a 5'-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA Topoisomerases
Alex B. Burgin, Jr

Isolation of Covalent Enzyme-DNA Complexes
Thomas C. Rowe, Dale Grabowski, and Ram Ganapathi

ICE Bioassay: Isolating In Vivo Complexes of Enzyme to DNA
Deepa Subramanian, Christine Sommer Furbee, and Mark T. Muller

DNA Unwinding Test Using Eukaryotic DNA Topoisomerase I
Linus L. Shen

Drug-DNA Interactions
David E. Graves

Quinolone Interactions with DNA and DNA Gyrase
Linus L.Shen

Bactericidal Assays for Fluoroquinolones
Thomas D. Gootz

Drug Toxicity in E. coli Cells Expressing Human DNA Topoisomerase I
Scott T. Taylor and Rolf Menzel

Drug-Induced Cytotoxicity in Tissue Culture
Cynthia E. Herzog and Leonard A. Zwelling

Analysis of Drug Uptake-Flow Cytometry
Grace Zoorob and Thomas Burke

Cell Cycle Analysis of Drug-Treated Cells
Frank Traganos, Gloria Juan, and Zbigniew Darzynkiewicz

Assaying Drug-Induced Apoptosis
Zbigniew Darzynkiewicz, Gloria Juan, and Frank Traganos

Single-Strand Conformational Polymorphism Analysis of DNA Topoisomerases I and II
Mary K. Danks

PCR-Based Cloning of DNA Topoisomerase Genes
Wai Mun Huang

Topoisomerase II-Catalyzed Relaxation and Catenation of Plasmid DNA
John M. Fortune and Neil Osheroff

Topoisomerase II-Mediated Cleavage of Plasmid DNA
D. Andrew Burden, Stacie J. Froelich-Ammon, and Neil Osheroff

Drug-induced Stabilization of Covalent DNA Topoisomerase I-DNA Intermediates: DNA Cleavage Assays
Paola Fiorani, Christine L. Hann, Piero Benedetti, and Mary-Ann Bjornsti

Studying DNA Topoisomerase I-Targeted Drugs in the Yeast Saccharomyces cerevisiae
Michael H. Woo, John R. Vance, and Mary-Ann Bjornsti

Yeast Systems for Demonstrating the Transfer of Antitopoisomerase II Agents
John L. Nitiss and Karen C. Nitiss


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