Downstream Processing of Proteins: Methods and Protocols / Edition 1

Downstream Processing of Proteins: Methods and Protocols / Edition 1

by Mohamed A. Desai
ISBN-10:
1617370754
ISBN-13:
9781617370755
Pub. Date:
11/19/2010
Publisher:
Springer-Verlag New York, LLC
Select a Purchase Option (Softcover reprint of hardcover 1st ed. 2000)
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Overview

Downstream Processing of Proteins: Methods and Protocols / Edition 1

Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also treated are various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Prools provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology.

Product Details

ISBN-13: 9781617370755
Publisher: Springer-Verlag New York, LLC
Publication date: 11/19/2010
Series: Methods in Biotechnology Series , #9
Edition description: Softcover reprint of hardcover 1st ed. 2000
Pages: 229
Product dimensions: 5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

Downstream Processing in the Biotechnology Industry: An Overview, Manohar Kalyanpur. Microbial Cell Disruption by High-Pressure Homogenization, Anton P. J. Middelberg. Protein Concentration and Buffer Exchange Using Ultrafiltration, Andrew L. Zydney and Ralf Kuriyel. High-Performance Tangential Flow Filtration for Protein Separations, Andrew L. Zydney and Ralf Kuriyel. Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation, Anton P. J. Middelberg. Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules, Mohamed A. Desai and Sandra P. Merino. Application of Chromatography in the Downstream Processing of Biomolecules, Mohamed A. Desai, Mark Rayner, Mike Burns, and Dervilla Bermingham. Affinity Ligand Technology: Optimal Protein Separations for Downstream Processing, Ken Jones and Dev Baines. Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules, Mohamed A. Desai. Expanded Bed Adsorption in the Purification of Biomolecules, Allan Lihme, Marie Hansen, Morten Olander, and Elias Zafirakos. Application of HPLC in the Purification of Biomolecules, Paul Bradley and Mohamed A. Desai. Countercurrent Chromatography of Proteins with Polymer Phase Systems, Yoichiro Shibusawa, Kazuhiro Matsuda, and Yoichiro Ito. Practical Aspects and Applications of Radial Flow Chromatography, Denise M. Wallworth. Sterile Filtration and Virus Filtration, Ralf Kuriyel and Andrew L. Zydney. Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products, Allan J. Darling. Validation of Sterilizing Filters in the Biotechnology Industry, Manohar Kalyanpur. Index.

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