Pub. Date:
Springer-Verlag New York, LLC
E. coli: Shiga Toxin Methods and Protocols / Edition 1

E. coli: Shiga Toxin Methods and Protocols / Edition 1

by Dana Philpott, Frank Ebel
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Product Details

ISBN-13: 9781617372643
Publisher: Springer-Verlag New York, LLC
Publication date: 10/08/2011
Series: Methods in Molecular Medicine , #73
Edition description: 2003
Pages: 352
Product dimensions: 5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections: An Overview
Mohamed A. Karmali

Methods for Detection of STEC in Humans: An Overview
James C. Paton and Adrienne W. Paton

Serological Methods for the Detection of STEC Infections
Martin Bitzan and Helge Karch

Detection and Characterization of STEC in Stool Samples Using PCR
Adrienne W. Paton and James C. Paton

Molecular Typing Methods for STEC
Haruo Watanabe, Jun Terajima, Hidemasa Izumiya, and Sunao Iyoda

STEC in the Food Chain: Methods for Detection of STEC in Food Samples
Michael Bülte

STEC as a Veterinary Problem: Diagnostics and Prophylaxis in Animals
Lothar H. Wieler and Rolf Bauerfeind

Cellular Microbiology of STEC Infections: An Overview
Frank Ebel and Dana Philpott

Analysis of Pathogenicity Islands of STEC
Tobias A. Oelschlaeger, Ulrich Dobrindt, Britta Janke, Barbara Middendorf, Helge Karch, and Jörg Hacker

Generation of Isogenic Deletion Mutants of STEC
Soudabeh Djafari, Nadja D. Hauf, and Judith F. Tyczka

Generation of Monoclonal Antibodies Against Secreted Proteins of STEC
Kirsten Niebuhr and Frank Ebel

Microscopic Methods to Study STEC: Analysis of the Attaching and Effacing Process
Stuart Knutton

Detection and Characterization of EHEC-Hemolysin
Herbert Schmidt and Roland Benz

Shiga Toxin Receptor Glycolipid Binding: Pathology and Utility
Clifford A. Lingwood

Methods for the Purification of Shiga Toxin 1
Anita Nutikka, Beth Binnington-Boyd, and Clifford A. Lingwood

Methods for the Identification of Host Receptors for Shiga Toxin
Anita Nutikka, Beth Binnington-Boyd, and Clifford A. Lingwood

Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport
Frédéric Mallard and Ludger Johannes

Measuring pHWithin the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin
Jae H. Kim

Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways
Nicola L. Jones

Interaction of Shiga Toxin with Endothelial Cells
Martin Bitzan and D. Maroeska W. M. te Loo

Shiga Toxin Interactions with the Intestinal Epithelium
Cheleste M. Thorpe, Bryan P. Hurley, and David W. K. Acheson

Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes
Christian Menge

Animal Models for STEC-Mediated Disease
Angela R. Melton-Celsa and Alison D. O'Brien

Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC
Florian Gunzer, Isabel Hennig-Pauka, Karl-Heinz Waldmann, and Michael Mengel

Bovine Escherichia coli O157: H7 Infection Model
Evelyn A. Dean-Nystrom


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