ISBN-10:
1583215832
ISBN-13:
9781583215838
Pub. Date:
03/22/2008
Publisher:
American Water Works Association
Early Detection of Cyanobacterial Toxins Using Genetic Methods

Early Detection of Cyanobacterial Toxins Using Genetic Methods

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Overview


This project examined the use of DNA-detection technology, in particular, real-time PCR, as a means of detecting toxic cyanobacteria in freshwaters. Research and development within the project was geared toward the delivery of a technology solution for the water industry where a real need was identified. A questionnaire was developed using a literature review that included DNA-detection technologies that might be suitable for the detection of waterborne microbes. End users polled on their requirements with 63 respondents, 75% from the United States and Australia. Results are discussed concluding that real-time PCR can be used effectively to detect and track changes in populations of toxic cyanobacteria but further work on the genetics of the toxic cyanobacteria will be necessary before the quantification by real-time PCR can be used to its fullest extent.

Product Details

ISBN-13: 9781583215838
Publisher: American Water Works Association
Publication date: 03/22/2008
Pages: 156

Table of Contents


List of Tables
List of Figures
Foreword
Acknowledgments

Executive Summary
Chapter 1: Literature Review
Chapter 2: Industry Questionnaire
Chapter 3: Elucidation of the Genetic Determinants of

Cylindrospermopsin Production
Chapter 4: Elucidation of the Genetic Determinants of Anatoxin-A Production
Chapter 5: Development of a Rapid Method for the Preparation of Cyanobacterial Samples
Chapter 6: Adaptation of Conventional PCR Assays to Real-Time PCR
Chapter 7: Testing of Portable Real-Time PCR in the Field
Chapter 8: Conclusions and Recommendations
Chapter 9: Materials and Methods
Appendix A: Questionnaire
Appendix B: Guide to Real-Time PCR Interpretation

References
Abbreviations

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