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Enzyme Kinetics: Principles and Methods
     

Enzyme Kinetics: Principles and Methods

by Hans Bisswanger, Leonie Bubenheim (Translator)
 

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This new, expanded and updated edition of the user-friendly and comprehensive treatise on enzyme kinetics expertly balances theory and practice. This is an indispensable aid for advanced students and professionals working with enzymes, whether biochemists, biotechnologists, chemical biologists, pharmacologists or bioengineers in academia, industry and clinical

Overview

This new, expanded and updated edition of the user-friendly and comprehensive treatise on enzyme kinetics expertly balances theory and practice. This is an indispensable aid for advanced students and professionals working with enzymes, whether biochemists, biotechnologists, chemical biologists, pharmacologists or bioengineers in academia, industry and clinical research.

Editorial Reviews

By applying techniques to pursue rapid kinetic reactions it is possible to separate a complete reaction into different time segments and to determine the rate constants of individual steps of the reaction. The author demonstrates the methods by which this is done and explains the theory behind it. Topics include reversible enzyme reactions, enzyme inhibition, multi-substrate reactions, special enzyme mechanisms, and isotope exchange. Specific methods detailed include electrochemical methods, calorimetry, and spectroscopy. The CD-ROM contains a graphics-plotting program intended as a companion to the book. Translated from the third edition of the German work Theorie und Methoden/>. Annotation c. Book News, Inc., Portland, OR (booknews.com)
From the Publisher
“Overall, this is an excellent and thorough treatment of a difficult subject. As well as appealing to biochemists, there are also elements of interest to those studying pharmacology and the pharmacokinetic distribution of drugs. The mathematical nature and advanced coverage means that it is most suitable for researchers who already have a strong grounding in the subject. I will certainly be consulting it during my research.”  (Biochemist, 29 May 2013)

“This new, expanded and updated edition of the user–friendly and comprehensive treatise on enzyme kinetics expertly balances theory and practice. This is an indispensable aid for advanced students and professionals working with enzymes, whether biochemists, biotechnologists, chemical biologists, pharmacologists or bioengineers in academia, industry and clinical research.”  (Biology Community, 29 November 2012)

Enzyme kinetics is not a new area in biochemistry. Thirty years ago, enzyme kinetics was one of the most important tools for deconstructing enzymatic mechanisms. With advances in enzyme structure determination and molecular genetics, enzyme kinetics is no longer as prominent. However, enzyme kinetics is still useful to gain insight into enzymes that are too large for NMR studies and that cannot be crystallized. Many enzymes that fit into this category are membrane bound, the kinetics of which are much more complicated. In this new edition (1st ed., 2002), Bisswanger (Univ. of Tübingen, Germany) does a nice job of extending solution enzyme kinetics to membrane-bound enzymes. Setting it apart from other works on the subject, Enzyme Kinetics does not simply deal with substrate binding as a part of the reaction kinetics, but instead devotes about one-third of the text to equilibrium binding between macromolecules and ligands in which no reaction catalysis follows the binding. This binding is then directly connected with enzyme catalyzed reaction kinetics. Enzyme Kinetics was written to serve as a graduate-level course resource and would serve this population well. The inclusion of student problems would be an improvement. Summing Up: Recommended. Graduate students, researchers, and faculty. — L. J. Liotta, Stonehill College (Choice, February 2009)

Product Details

ISBN-13:
9783527303434
Publisher:
Wiley
Publication date:
04/08/2002
Edition description:
BK&CD-ROM
Pages:
260
Product dimensions:
6.99(w) x 9.70(h) x 0.58(d)

Meet the Author

Hans Bisswanger was Professor at the Interfaculty Institute of Biochemistry at the University of Tübingen (Germany), where he has developed and taught for many years an intensive course on enzyme kinetics, enzyme technology and ligand binding. His scientific interest lies with structural and regulatory mechanisms of multi-enzyme complexes, thermophilic enzymes and the technical application of immobilized enzymes. He is the author of two well-known books on enzymology that have appeared in different languages and editions.

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