Enzymes as Sensors

Enzymes as Sensors

by Elsevier Science


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Product Details

ISBN-13: 9780128054062
Publisher: Elsevier Science
Publication date: 04/07/2017
Series: Methods in Enzymology Series , #589
Pages: 530
Product dimensions: 6.00(w) x 9.00(h) x (d)

About the Author

Richard B. Thompson is Associate Professor at Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, U.S.A.

Carol Fierke is Professor and Chair at the Department of Chemistry and Professor of Biological Chemistry at the Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, U.S.A.

Table of Contents

1. Recent Advances in Development of Genetically Encoded Fluorescent Sensors 2. Engineering Rugged Field Assays to Detect Hazardous Chemicals Using Spore-Based Bacterial Biosensors 3. Engineering BRET-Sensor Proteins 4. Enzymes as Sensors 5. Integrated Strategies to Gain a Systems-Level View of Dynamic Signaling Networks 6. Probing Cdc42 Polarization Dynamics in Budding Yeast Using a Biosensor 7. Novel Fluorescence-Based Biosensors Incorporating Unnatural Amino Acids 8. Folding- and Dynamics-Based Electrochemical DNA Sensors 9. Construction of Protein-Based Biosensors Using Ligand-Directed Chemistry for Detecting Analyte Binding 10. Measuring and Imaging Metal Ions With Fluorescence-Based Biosensors: Speciation, Selectivity, Kinetics, and Other Issues 11. Bioapplications of Electrochemical Sensors and Biosensors 12. A Highly Sensitive Biosensor for ATP Using a Chimeric Firefly Luciferase 13. Highly Modular Bioluminescent Sensors for Small Molecules and Proteins 14. Sensitive Protein Detection and Quantification in Paper-Based Microfluidics for the Point of Care 15. Microneedle Enzyme Sensor Arrays for Continuous In Vivo Monitoring 16. Visualization of the Genomic Loci That Are Bound by Specific Multiprotein Complexes by Bimolecular Fluorescence Complementation Analysis on Drosophila Polytene Chromosomes

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