Germline Development: Methods and Protocols

Germline Development: Methods and Protocols


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Product Details

ISBN-13: 9781617794353
Publisher: Springer-Verlag New York, LLC
Publication date: 12/06/2011
Series: Methods in Molecular Biology , #825
Edition description: 2012
Pages: 256
Product dimensions: 7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

Part I: Male Germline

1. Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular Studies
Yunmin Li, Teruko Taketo, and Yun-Fai Chris Lau

2. Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical Analysis
Gurpreet Manku, Monty Mazer, and Martine Culty

3. Isolation of Undifferentiated and Early Differentiating Type A Spermatogonia from Pou5f1-GFP Reporter Mice
Thomas Garcia and Marie-Claude Hofmann

4. Isolation of Human Male Germline Stem Cells Using Enzymatic Digestion and Magnetic-Activated Cell Sorting
Zuping He, Maria Kokkinaki, Jiji Jiang, Wenxian Zeng, Ina Dobrinski, and Martin Dym

5. Isolation and Purification of Murine Male Germ Cells
Catherine Boucheron and Vanessa Baxendale

6. Preparation of Enriched Mouse Syncitia-Free Pachytene Spermatocyte Cell Suspensions
Yan Wang and Martine Culty

7. Revealing Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray
Tin-Lap Lee, Owen M. Rennert, and Wai-Yee Chan

8. Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase
Alan Lap-Yin Pang

9. Identification of Novel Long Non-Coding RNA Transcripts in Male Germ Cells
Tin-Lap Lee, Amy Xiao, and Owen M. Rennert

10. Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization
Hoi-Hung Cheung, Tin-Lap Lee, Owen M. Rennert, and Wai-Yee Chan

11. Spermatogenesis in Cryptorchidism
Alexander I. Agoulnik, Zaohua Huang, and Lydia Ferguson

Part II: Female Germline

12. In Vitro Culture of Fetal Ovaries: A Model to Study Factors Regulating Early Follicular Development
Shyamal K. Roy, Cheng Wang, Anindit Mukherjee, and Prabuddha Chakraborty

13. Microspread Oocyte Preparations for the Analysis of Meiotic Prophase Progression with Improved Recovery by Cytospin Centrifugation
Teruko Taketo

14. In Vitro Maturation (IVM) of Porcine Oocytes
Ye Yuan and Rebecca L. Krisher

15. Experimental Approaches to the Study of Human Primordial Germ Cells
Andrew J. Childs and Richard A. Anderson

16. Investigating the Origins of Somatic Cell Populations in the Perinatal Mouse Ovaries Using Genetic Lineage Tracing and Immunohistochemistry
Chang Liu, Melissa Paczkowski, Manal Othman, and Humphrey Hung-Chang Yao

17. DNA Methylation Analysis of Germ Cells by Using Bisulfite-Based Sequencing Methods
Hisato Kobayashi and Tomohiro Kono

18. Gene Expression in Mouse Oocytes by RNA-Seq
Eric Antoniou and Robert Taft

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