Pub. Date:
Springer-Verlag New York, LLC
Human Airway Inflammation: Sampling Techniques and Analytical Protocols

Human Airway Inflammation: Sampling Techniques and Analytical Protocols

by Duncan F. Rogers, Louise E. Donnelly


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Rubor (redness), tumor (swelling), calor (heat), and dolor (pain) are the classical signs of inflammation. These features are obvious in the skin, where injury or disease causes flare, wheal, and painful burning sensations. Vasodi- tation underlies the flare and heat, plasma exudation the swelling, and acti- tion of sensory nerves relays pain. In chronic conditions, skin biopsies show inflammatory cell infiltrate. Inflammation is not unique to the skin and contr- utes to disease and repair processes in other organ systems in the body. From the viewpoint of this volume, lung inflammation is now recognized as central to the pathophysiology of a number of severe respiratory conditions, the two most common being asthma and chronic obstructive pulmonary disease (COPD). In asthma, and to a lesser extent COPD, there is evidence of vasodilatation, with congestion of blood vessels accompanied by reddening of the airway mucosa, and of plasma exudation, leading to swelling of the airway wall. Similarly, although less pronounced than in the skin, there is evidence of pain, for example, the - pleasant chest sensations associated with asthma attacks. Understanding the pat- genesis of airway inflammation will enable rational design of drugs to effectively treat conditions such as asthma and COPD. However, whereas immediate access to the skin facilitates investigation of disease processes, the lung, although “open to atmosphere,” is much less accessible. Consequently, the investigation of lung inflammation is usually indirect. Thus, a wide variety of research techniques are used.

Product Details

ISBN-13: 9781489943729
Publisher: Springer-Verlag New York, LLC
Publication date: 08/09/2013
Series: Methods in Molecular Medicine , #56
Edition description: Softcover reprint of the original 1st ed. 2001
Pages: 440
Product dimensions: 5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

Airway Inflammation and Remodeling in Asthma: Current Concepts
Stephen T. Holgate

Part I. Sample Collection: Invasive Techniques

Biopsy Techniques: Optimization for Collection and Preservatio
Marina Saetta and Graziella Turato

Bronchoalveolar Lavage (BAL): Critical Evaluation of Techniques
Christopher Ward and E. Haydn Walters

Nasal Secretions and Exudations: Collection and Approaches to Analysis
Lennard Greiff, Morgan Andersson, and Carl G.A. Persson

Part II. Sample Collection: Noninvasive Techniques

Induced Sputum: Selection Method
Margaret M. Kelly, Ann Efthimiadis, and Frederick E. Hargreave

Induced Sputum: Whole Sample
Vera M. Keatings and Julia A. Nightingale

Measurement of Exhaled Nitric Oxide and Carbon Monoxide
Sergei A. Kharitonov

Measurement of Exhaled Hydrocarbons
Paolo Paredi, Sergei A. Kharitonov, and Peter J. Barnes

Breath Condensate as a Vehicle for Collection of Inflammatory Mediators, Especially Hydrogen Peroxide
Johan C. de Jongste, Rijn Jöbsis, and H. Rolien Raatgeep

Part III. Cell Isolation and Culture

Airway Epithelial Cells (Primaries vs Cell Lines)
Louise E. Donnelly

Isolation and Culture of Human Alveolar Type II Pneumocytes
Ian R. Witherden and Teresa D. Tetley

Pulmonary Artery Endothelial Cells
Joachim Seybold and Norbert Suttorp

Isolation and Culture of Human Airway Smooth Muscle Cells
Aili L. Lazaar and Reynold A. Panettieri, Jr

Isolation and Purification of Human Mast Cells and Basophils
Bernhard F. Gibbs and Madeleine Ennis

Neutrophils: Collection, Separation, and Activation
Sarah V. Culpitt

Macrophages: Identification, Separation, and Function
Leonard W. Poulter and C.M. Burke

Collection, Separation, and Activation of Human T Lymphocytes
YannisSotsios and Stephen G. Ward

Eosinophils: Collection, Separation, and Activation
Leo Koenderman, Jan van der Linden, Laurien Ulfman, and Paul Coffer

Part IV. Gene Expression/Regulation

Analysis of Gene Expression: Reverse Transcription-Polymerase Chain Reaction
El-Bdaoui Haddad and Jonathan Roussell

Analysis of Gene Expression: Northern Blotting
Jonathan Roussell and El-Bdaoui Haddad

The Technique of In Situ Hybridization
Sun Ying and A. Barry Kay

Measurement of Airway Mucin Gene Expression
Kelly Pritchard, Alinka K. Smith, and Duncan F. Rogers

Transcription Factors and Inflammatory Gene Regulation: Strategic Approaches
Robert Newton and Karl J. Staples

Part V. Mediators: Expression and Function

Inflammatory Mediators in Spontaneously Produced Sputum
Adam Hill, Simon Gompertz, Darren Bayley, and Robert Stockley

Flow Cytometry: Measurement of Leukocyte Receptor Expression and Function
Adele Hartnell

Measurement of Granulocyte Pharmacodynamics in Whole Blood by Flow Cytometry
Shannon A. Bryan, Margaret J. Leckie, Gavin Jenkins, Peter J. Barnes, Timothy J. Williams, Ian Sabroe, and Trevor T. Hansel

Current In Vitro Models of Leukocyte Migration: Methods and Interpretation
Jennifer R. Allport, Guillermo Garcia-Cardeña, Yaw-Chyn Lim, and Francis W. Luscinskas

Tracing Intracellular Mediator Storage and Mobilization in Eosinophils
Salahaddin Mahmudi-Azer, Paige Lacey, and Redwan Moqbel

Measurement of Metalloproteinases
Steven D. Shapiro, Diane Kelley and Dale Kobayashi

Immunocytochemistry and Immunohistochemistry
Jochen Springer and Axel Fischer

The Measurement of Cysteinyl Leukotrienes in Urine
Jay Y. Wescott and Sally E. Wenzel

Quantitative Analysis of Cyclooxygenase Metabolites of Arachidonic Acid

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