Pub. Date:
Springer-Verlag New York, LLC
Human Retrovirus Protocols: Virology and Molecular Biology / Edition 1

Human Retrovirus Protocols: Virology and Molecular Biology / Edition 1

by Tuofu Zhu
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A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.

Product Details

ISBN-13: 9781617375996
Publisher: Springer-Verlag New York, LLC
Publication date: 11/19/2010
Series: Methods in Molecular Biology , #304
Edition description: Softcover reprint of hardcover 1st ed. 2005
Pages: 494
Product dimensions: 5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

Part I. Methods for Virus Isolation and Detection

Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals
Janet D. Siliciano and Robert F. Siliciano

Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals
Hanneke Schuitemaker and Neeltje A. Kootstra

Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes
Paul R. Gorry, Secondo Sonza, Katherine Kedzierska, and Suzanne M. Crowe

Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid
Santhi Gorantla, Myhanh Che, and Howard E. Gendelman

Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue
Kathleen Borgmann, Howard E. Gendelman, and Anuja Ghorpade

Isolation of Human Immunodeficiency Virus Type 1 From Semen and Vaginal Fluids
Ann A. Kiessling

Isolation and Quantification of HIV From Lymph Nodes
Catherine Tamalet

Isolation of Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes
Hetty Blaak

Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells
Michael D. Lairmore and Andy Montgomery

Isolation of Foamy Viruses From Peripheral Blood Lymphocytes
Joëlle Tobaly-Tapiero, Patricia Bittoun, and Ali Saïb

Alu-LTR Real-Time Nested PCR Assay for Quantifying Integrated HIV-1 DNA
Audrey Brussel, Olivier Delelis, and Pierre Sonigo

Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR
Olivier Delelis, Audrey Brussel, and Pierre Sonigo

Detection of HIV-1 Provirus and RNA by In Situ Amplification
Alcina Nicol and Gerard J.Nuovo

Detection of HTLV-1 Gene on Cytologic Smear Slides
Kenji Kashima, Tsutomu Daa, and Shigeo Yokoyama

Detection of HIV-2 by PCR
Feng Gao

Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretions
Susan A. Fiscus

Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR
Marie Gueudin, Florence Damond, and François Simon

Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR
Marie Gueudin and François Simon

A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay
Susanne Polywka, Hedwig Duttmann, Frank Lübben, Rainer Laufs, and Jürgen Feldner

A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity
Bernard Weber

Detection of Antiviral Antibodies Using Enzyme-Linked Immunosorbent Assay
Ingrid G. Winkler

Plaque-Reduction Assays for Human and Simian Immunodeficiency Virus Neutralization
Anna Nordqvist and Eva Maria Fenyö

Detection of Drug-Resistant HIV-1 Strains
Ann A. Kiessling, S. J. Eyre, and B. D. Desmarais

Part II. Molecular Biology Methods

Determination of Cell Tropism of HIV-1
Neeltje A. Kootstra and Hanneke Schuitemaker

Determination of Co-Receptor Usage of HIV-1
Hanneke Schuitemaker and Neeltje A. Kootstra

Quantitative Evaluation of HIV and SIV Co-Receptor Use With GHOST(3) Cell Assay
Dalma Vödrös and Eva Maria Fenyö

Phenotypic Characterization of Blood Monocytes From HIV-Infected Individuals
Philip J. Ellery and Suzanne M. Crowe

Methods to Determine HIV-1 Ex Vivo Fitness
Awet Abraha, Ryan M. Troyer, Miguel E. Quiñones-Mateu, and Eric J. Arts

A Yeast Recombination-Based Cloning System to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes

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