ISBN-10:
1617374733
ISBN-13:
9781617374739
Pub. Date:
11/19/2010
Publisher:
Springer-Verlag New York, LLC
Immobilization of Enzymes and Cells / Edition 2

Immobilization of Enzymes and Cells / Edition 2

by José M. Guisán
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Overview

Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective prools, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of prools for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few prools are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.

Product Details

ISBN-13: 9781617374739
Publisher: Springer-Verlag New York, LLC
Publication date: 11/19/2010
Series: Methods in Biotechnology , #22
Edition description: Softcover reprint of hardcover 2nd ed. 2006
Pages: 450
Product dimensions: 5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

Immobilization of Enzymes as the 21st Century Begins: An Already Solved Problem or Still an Exciting Challenge?
Jose M. Guisan

Immobilization of Enzymes: A Literature Survey
Beatriz M. Brena and Franciso Batista-Viera

Cross-Linked Enzyme Aggregates
Roger A. Sheldon, Rob Schoevaart, and Luuk M. van Langen

Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups
Cesar Mateo, Olga Abian, Gloria Fernández-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan, and Roberto Fernandez-Lafuente

Glutaraldehyde in Protein Immobilization: A Versatile Reagent
Lorena Betancor, Fernando L—pez-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente, and Jose M. Guisan

Practical Prools for Lipase Immobilization Via Sol-Gel Techniques
Manfred T. Reetz

Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques
Mònica Campàs and Jean-Louis Marty

Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers
Ipsita Roy and Munishwar N. Gupta

Affinity Immobilization of Tagged Enzymes
Silvana Andreescu, Bogdan Bucur, and Jean-Louis Marty

Bioaffinity Immobilization
Ipsita Roy and Munishwar N. Gupta

One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports
Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando L—pez-Gallego, Jose M. Guisan, and Roberto Fernandez-Lafuente

Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran
Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo, Gloria Fernández-Lorente, Roberto Fernandez-Lafuente, and Jose M. Guisan

Purification, Immobilization,Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports
Jose M. Palomo, Gloria Fernández-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente, and Jose M. Guisan

Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads®
Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando L—pez-Gallego, Rodrigo Torres, Manuel Fuentes, Aurelio Hidalgo, Jose M. Palomo, Lorena Betancor, Gloria Fernández-Lorente, Claudia Ortiz, Olga Abian, Jose M. Guisan, and Roberto Fernandez-Lafuente

Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports
Tamara Montes, Fernando L—pez-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor, Jose M. Guisan, and Roberto Fernandez-Lafuente

Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach
Alankar Vaidya and Lutz Fischer

Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups
Francisco Batista-Viera, Karen Ovsejevi, and Carmen Manta

Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers
Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando L—pez-Gallego, Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan, and Roberto Fernandez-Lafuente

Immobilization of Enzymes on Magnetic Particles
Martina Koneracká, Peter Kopcansky , Milan Timko, Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan, and Anil de Sequeira

Immobilization of Proteins on Gold Surfaces
José M. Abad, Marcos Pita, and Víctor M. Fernández

Immobilization of Enzymes on Electrodes
Gilvanda Silva Nunes and Jean-Louis Marty

Immobilization of Enzymes for Use in Organic Media
Patrick

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