Immune Receptors: Methods and Protocols

Immune Receptors: Methods and Protocols

Hardcover(2011)

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Overview

Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity. The receptors that mediate these functions are at the front lines of both protective and regulative roles of the immune system. In Immune Receptors: Methods and Prools, expert researchers present a variety of experimental approaches to the characterization of immune receptors and the cell biology that mediates their functions. These include imaging techniques that aim to understand receptor localization and trafficking, techniques to measure receptor-ligand interactions, strategies to identify novel ligands and methods to analyze downstream receptor signaling,as well as strategies for genomic and proteomic characterization of receptor repertoires. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Immune Receptors: Methods and Prools offers technical descriptions and prools that will be useful both to investigators who are interested in carrying out these procedures and to those who seek a deeper understanding of the bench science that lies behind the immunology literature.

Product Details

ISBN-13: 9781617791383
Publisher: Springer-Verlag New York, LLC
Publication date: 06/29/2011
Series: Methods in Molecular Biology , #748
Edition description: 2011
Pages: 301
Product dimensions: 7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

1. Screening for Ligands of C-Type Lectin-Like Receptors

Elwira Pyż and Gordon D. Brown

2. Yeast Surface Display of Lamprey Variable Lymphocyte Receptors (VLR)

Gang Xu, Satoshi Tasumi, and Zeev Pancer

3. Identification of Scavenger Receptor Ligands

Claudine Neyen, Annette Pluddemann, and Siamon Gordon

4. Construction, Expression, and Purification of Chimeric Protein Reagents Based on Immunoglobulin Fc Regions

John P. Cannon, Marci O’Driscoll, and Gary W. Litman

5. Innate Immune Receptors for Nucleic Acids

Andrea Stutz, Damien Bertheloot, and Eicke Latz

6. Analysis of Receptor-Ligand Interactions by Surface Plasmon Resonance

Kimiko Kuroki and Katsumi Maenaka

7. Cell-Based Reporter Assay to Analyze Activation of Nod1 and Nod2

Birte Zurek, Harald Bielig, and Thomas A. Kufer

8. Determining FcεRI Diffusional Dynamics Via Single Quantum Dot Tracking

Diane S. Lidke, Shalini T. Low-Nam, Patrick J. Cutler, and Keith A. Lidke

9. Ratiometric Analysis of Subcellular Recruitment of Fc Receptors During Phagocytosis

Patricia Mero and James W.D. Booth

10. Assessment of the Recycling of the Membrane-Bound Chemokine, CX3CL1

Sajedabanu Patel, Ilya Mukovozov, and Lisa A. Robinson

11. Measuring Immune Receptor Mobility by Fluorescence Recovery after Photobleaching

Kristen Silver and Rene E. Harrison

12. Probing the Plasma Membrane Structure of Immune Cells through the Analysis of Membrane Sheets by Electron Microscopy

Björn F. Lillemeier and Mark M. Davis

13. Rapamycin-Based Inducible Translocation Systems for Studying Phagocytosis

Michal Bohdanowicz and Gregory D. Fairn

14. Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling

Alexis J. Torres, David Holowka, and Barbara A. Baird

15. CELLISA: Reporter Cell-Based Immunization and Screening of Hybridomas Specific for Cell Surface Antigens

Peter Chen, Aruz Mesci, and James R. Carlyle

16. Use of Colloidal Silica-Beads for the Isolation of Cell-Surface Proteins for Mass Spectrometry-Based Proteomics

Yunee Kim, Sarah Elschenbroich, Parveen Sharma, Lusia Sepiashvili, Anthony O. Gramolini, and Thomas Kislinger

17. Transfection-Based Genomic Readout for Identifying Rare Transcriptional Splice Variants

Larry J. Dishaw, M. Gail Mueller, Robert N. Haire, and Gary W. Litman

18. Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System

Nagarama Kothapalli and Sebastian D. Fugmann

19. Characterizing Immune Receptors from New Genome Sequences

Katherine M. Buckley and Jonathan P. Rast

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