ISBN-10:
160761782X
ISBN-13:
9781607617822
Pub. Date:
07/20/2010
Publisher:
Springer-Verlag New York, LLC
Immunoelectron Microscopy: Methods and Protocols / Edition 1

Immunoelectron Microscopy: Methods and Protocols / Edition 1

by Steven D. Schwartzbach, Tetsuaki Osafune

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Product Details

ISBN-13: 9781607617822
Publisher: Springer-Verlag New York, LLC
Publication date: 07/20/2010
Series: Methods in Molecular Biology , #657
Edition description: 2010
Pages: 352
Product dimensions: 7.20(w) x 10.20(h) x 1.00(d)

Table of Contents

Part I: Molecular Toolbox

1. Protein Antigen Expression in E. coli for Antibody Production David M. Rancour, Steven K. Backues, and Sebastian Y. Bednarek

2. Expression of Epitope-Tagged Proteins in Plants Takuya Furuichi

3. Expression of Epitope-Tagged Proteins in Arabidopsis Leaf Mesophyll Protoplasts Young-Hee Cho and Sang-Dong Yoo

4. Transient Expression of Epitope-Tagged Proteins in Mammalian Cells Melanie L. Styers, Jason Lowery, and Elizabeth Sztul

5. Production and Purification of Polyclonal Antibodies Masami Nakazawa, Mari Mukumoto, and Kazutaka Miyatake

6. Production and Purification of Monoclonal Antibodies Masami Nakazawa, Mari Mukumoto, and Kazutaka Miyatake

7. Production of Antipetide Antibodies Bao-Shiang Lee, Jin-Sheng Huang, G.D. Lasanthi P. Jayathilaka, Syed S. Lateef, and Shalini Gupta

8. Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab’)2 and Strepavidin Sadaki Yokota

Part II: Microscopy Toolbox

9. Immunoelectron Microscopy of Chemically Fixed Developing Plant Embryos Tetsuaki Osafune and Steven D. Schwartzbach

10. Pre-Embedding Immunogold Localization of Antigens in Mammalian Brain Slices Thomas Schikorski

11. Pre-Embedding Immunoelectron Microscopy of Chemically Fixed Mammalian Tissue Culture Cells Haruo Hagiwara, Takeo Aoki, Takeshi Suzuki, and Kuniaki Takata

12. Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues Miyuki Takeuchi, Keiji Takabe, and Yoshinobu Mineyuki

13. In vivo Cryotechniques for Preparation of Animal Tissues for Immunoelectron Microscopy Shinichi Ohno, Nobuhiko Ohno, Nobuo Terada, Sei Saitoh, Yurika Saitoh, and Yasuhisa Fujii

14. Immunoelectron Microscopy of Cryofixed Freeze Substituted Mammalian Tissue Culture Cells Akira Sawaguchi

15. Immunoelectron Microscopy of Cryofixed Freeze Substituted Saccharomyces cerevisiae Jindriska Fiserova and Martin W. Goldberg

16. High Resolution Molecular Localization by Freeze-Fracture Replica Labeling Akikazu Fujita and Toyoshi Fujimoto

17. Pre-Embedding Electron Microscopy Methods for Glycan Localization in Chemically Fixed Mammalian Tissue Using Horseradish Peroxidase-Conjugated Lectin Yoshihiro Akimoto and Hayato Kawakami

18. Pre-Embedding Nanogold Silver and Gold Intensification Akitsugu Yamamoto and Ryuichi Masaki

19. The Post-Embedding Method for Immunoelectron Microscopy of Mammalian Tissues: A Standardized Procedure Based on Heat-Induced Antigen Retrieval Shuji Yamashita

20. Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells Haruo Hagiwara, Takeo Aoki, Takeshi Suzuki, and Kuniaki Takata

21. Serial Section Immunoelectron Microscopy of Algal Cells Tetsuaki Osafune and Steven D. Schwartzbach

22. Freeze-Etch Electron Tomography for the Plasma Membrane Interface Nobuhiro Morone

23. Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy Seiichi Sato and Yasushi Sato

24. Immunogold Labeling for Scanning Electron Microscopy Martin W. Goldberg and Jindriska Fiserova

25. Horseradish Peroxidase as a Reporter Gene and as a Cell-Organelle-Specific Marker in Correlative Light-Electron Microscopy Thomas Schikorski

26. Monitoring Rapid Endocytosis in the Electron Microscope via Phoonversion of Vesicles Fluorescently Labeled with FM1-43
Thomas Schikorski

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