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Elsevier Science
Immunology of Infection / Edition 3

Immunology of Infection / Edition 3

by Elsevier Science


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Product Details

ISBN-13: 9780123748423
Publisher: Elsevier Science
Publication date: 11/15/2010
Series: Methods in Microbiology Series
Pages: 544
Product dimensions: 7.30(w) x 9.40(h) x 1.20(d)

Table of Contents

Preface to the 2nd edition
Preface to the 1st edition

The immune response to infectious agents

General Methods
Phenotyping and separation of leukocyte populations based on affinity labelling
Cytometric cytokine secretion assay: detection and isolation of cytokine secreting T cells
Measurement of cellular proliferation
Use of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine design
The use of tetramers in the quantitative analysis of T cell responses
Using microarrays for studying the host transcriptional response to microbial infection
Genome-wide expression profiling of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophages

Murine Models
Management of immuno-compromised and infected animals
In Vitro Analysis
Isolation and preparation of lymphocytes from infected animals for in vitro analysis
Isolation and development of murine T-lymphocyte hybridomas and clonal cell lines
Killer cell assays
Quantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR Methods
Isolation of and measuring the function of professional phagocytes: murine macrophages
In vivo Analysis
The immune response in mice challenged with bacterial infections
Measuring immune responses in vivo
Specific Models
Murine model of tuberculosis
The leishmaniasis model
Animal models: murine cytomegalovirus
DNA vaccines: fundamentals and practice
Preparation and use of adjuvants

Human Systems
Isolation and propagation of human dendritic cells
Isolation of T cells and establishment of T-cell lines and clones
Growth-transformation of human T cells
Generation and characterisation of human killer cells
Measuring human cytokine responses
Measuring immune responses in situ:
immunofluorescent and immunoenzymatic techniques
Isolation, characterisation and cultivation of human monocytes and macrophages

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