Pub. Date:
Springer New York
Immunophenotyping: Methods and Protocols

Immunophenotyping: Methods and Protocols

by J. Philip McCoy, Jr


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This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immunophenotyping: Methods and Prools is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.

Product Details

ISBN-13: 9781493996490
Publisher: Springer New York
Publication date: 09/15/2019
Series: Methods in Molecular Biology , #2032
Edition description: 1st ed. 2019
Pages: 356
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Table of Contents…
Contributing Authors…

1. High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook
Florian Mair and Aaron J. Tyznik

2. Immunophenotyping by Mass Cytometry
Gregory K. Behbehani

3. Fluorescent Cell Barcoding for Immunophenotyping
Valentina Giudice. Giovanna Fantoni, and Angélique Biancotto

4. Immunophenotyping using Dried and Lyophilized Reagents
Marc Langweiler

5. Guidelines for Gating Flow Cytometry Data for Immunological Assays
Janet Staats, Anagha Divekar, J. Philip McCoy, and Holden T. Maecker

6. Strategies and Techniques for NK Cell Phenotyping
Chen Ziqing, Andreas Lundqvist, and Kristina Witt

7. Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue
Alain Diaz, Maria Romero, Daniela Frasca, and Bonnie B. Blomberg

8. Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells
Ankit Saxena, Pradeep K. Dagur, and Angélique Biancotto

9. Immunophenotyping of Human Regulatory T Cells
Janet Staats

10. Immunophenotyping of Human Innate Lymphoid Cells
Sara Trabanelli, Alejandra Gomez-Cadena, and Camilla Jandus

11. Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis
Abdullah Alsuwaidan, Franklin Fuda, Weina Chen, and Mingyi Chen

12. Immunophenotyping of Circulating Endothelial Cell and Endothelial MicroparticlesNicholas Wanner and Kewal Asosingh

13. Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells
Moen Sen, Ling Wang, Liping Yu, and Erica L. Carpenter

14. Quality Control of Immunophenotyping
Bruce Greig

15. Immunophenotyping of Acute Myeloid Leukemia
Pallavi Kanwar Galera, Chunjie Jiang, and Raul Braylan

16. Immunophenotyping of Acute Lymphoblastic Leukemia
Joseph A. DiGiuseppe and Jolene L. Cadinali

17. Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia
Dalia A. Salem and Maryalice Stetler-Stevenson

18. Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH)
Andrea J. Illingworth, Iuri Marinov, and D. Robert Sutherland

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