Kinase C Probes

Kinase C Probes

by Andreas Schleifenbaum
Pub. Date:
VDM Verlag Dr. Mueller e.K.


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Kinase C Probes

Understanding cellular function is a pivotal aim in the life sciences. Monitoring the interplay of regulatory proteins illuminates cellular function on a molecular level. One powerful method is fluorescence microscopy, which enables the spatio-temporal resolution of cellular events. To this end, several fluorescent reporters can be monitored within a single cell and interdependency between these factors can be determined.

This book presents fluorescent reporters for monitoring protein kinase C, a central actor on the stage of cellular signalling. Aspects ranging from probe design to the evaluation of experimental results are discussed. Further, experiments designed for high-throughput screening of new probe variants or PKC-effector molecules are described, including methods for visualizing and judging the observed data. Finally, the probe's capacity for use in multiparameter experiments is demonstrated.

This book is complemented by a detailed experimental part including a flexible cloning system for rapid probe development and computer scripts supporting the analysis of experimental data.

This book is directed to researchers interested in fluorescence probe development.

Product Details

ISBN-13: 9783639036510
Publisher: VDM Verlag Dr. Mueller e.K.
Publication date: 07/20/2008
Pages: 156
Product dimensions: 6.00(w) x 9.00(h) x 0.33(d)

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