Kinase C Probes available in Paperback
- Pub. Date:
- VDM Verlag Dr. Mueller e.K.
Understanding cellular function is a pivotal aim in the life sciences. Monitoring the interplay of regulatory proteins illuminates cellular function on a molecular level. One powerful method is fluorescence microscopy, which enables the spatio-temporal resolution of cellular events. To this end, several fluorescent reporters can be monitored within a single cell and interdependency between these factors can be determined.
This book presents fluorescent reporters for monitoring protein kinase C, a central actor on the stage of cellular signalling. Aspects ranging from probe design to the evaluation of experimental results are discussed. Further, experiments designed for high-throughput screening of new probe variants or PKC-effector molecules are described, including methods for visualizing and judging the observed data. Finally, the probe's capacity for use in multiparameter experiments is demonstrated.
This book is complemented by a detailed experimental part including a flexible cloning system for rapid probe development and computer scripts supporting the analysis of experimental data.
This book is directed to researchers interested in fluorescence probe development.
|Publisher:||VDM Verlag Dr. Mueller e.K.|
|Product dimensions:||6.00(w) x 9.00(h) x 0.33(d)|