ISBN-10:
0805384529
ISBN-13:
9780805384529
Pub. Date:
10/28/1997
Publisher:
Benjamin-Cummings Publishing Company
Laboratory Experiments in Microbiology / Edition 6

Laboratory Experiments in Microbiology / Edition 6

by Ted R. Johnson

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Product Details

ISBN-13: 9780805384529
Publisher: Benjamin-Cummings Publishing Company
Publication date: 10/28/1997
Edition description: Older Edition
Pages: 418
Product dimensions: 9.04(w) x 10.68(h) x 0.94(d)

About the Author

Ted R. Johnson is a professor emeritus of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he has taught courses in microbiology and immunology. He has taught at St. Olaf for 39 years and previously taught at Mankato State University for five years. He received his master’s degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several semester abroad and interim programs. He has been involved in advising health professions students and received the Fr.Joseph Walter Award for Health Profession advising. His research focuses on the immune response to virally induced cancer in various animal models.

Christine L. Case is a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 47 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.




Table of Contents

Introduction.

I. MICROSCOPY.

1. Use and Care of the Microscope.
2. Examination of Living Microorganisms.

II. STAINING METHODS.

3. Preparation of Smears and Simple Staining.
4. Negative Staining.
5. Gram Staining.
6. Acid-fast Staining.
7. Structural Stains (Endospore, Capsule, and Flagella).
8. Morphologic Unknown.

III. CULTIVATION OF BACTERIA.

9. Microbes in the Environment.
10. Transfer of Bacteria: Aseptic Technique.
11. Isolation of Bacteria by Dilution Techniques.
12. Special Media for Isolating Bacteria.

IV. MICROBIAL METABOLISM.

13. Carbohydrate Catabolism.
14. Fermentation of Carbohydrates.
15. Protein Catabolism, Part 1.
16. Protein Catabolism, Part 2.
17. Respiration.
18. Rapid Identification Methods.

V. MICROBIAL GROWTH.

19. Oxygen and the Growth of Bacteria.
20. Determination of a Bacterial Growth Curve: The Role of Temperature.
21. Other Influences on Microbial Growth: Osmotic Pressure and pH.

VI. CONTROL OF MICROBIAL GROWTH.

22. Physical Methods of Control: Heat.
23.Physical Methods of Control: Ultraviolet Radiation.
24. Chemical Methods of Control: Disinfectants and Antiseptics.
25. Chemical Methods of Control: Antimicrobial Drugs.
26. Effectiveness of Hand Scrubbing.

VII. MICROBIAL GENETICS.

27. Regulation of Gene Expression.
28. Isolation of Bacterial Mutants.
29. Transformation of Bacteria.
30. DNA Fingerprinting.
31. Genetic Engineering.
32. Ames Test for Detecting Possible Chemical Carcinogens.

VIII. THE MICROBIAL WORLD.

33. Unknown Identification and Bergey's Manual.
34. Fungi: Yeasts.
35. Fungi: Molds.
36. Phototrophs: Algae and Cyanobacteria.
37. Protozoa.

IX. VIRUSES.

38. Isolation and Titration of Bacteriophages.
39. Plant Viruses.

X: INTERACTION OF MICROBE AND HOST.

40. Epidemiology.
41. Koch's Postulates.

XI. IMMUNOLOGY.

42. Nonspecific Resistance.
43. Blood Group Determination: Slide Agglutination.
44. Agglutination Reactions: Microtiter Agglutination.
45. ELISA Technique.

XII. MICROORGANISMS AND DISEASE.

46. Bacteria of the Skin.
47. Bacteria of the Respiratory Tract.
48. Bacteria of the Mouth.
49. Bacteria of the Gastrointestinal Tract.
50. Bacteria of the Urogenital Tract.
51. Identification of an Unknown from a Clinical Sample.

XIII. MICROBIOLOGY AND THE ENVIRONMENT.

52. Microbes in Water: Multiple-Tube Technique.
53. Microbes in Water: Membrane Filter Technique.
54. Microbes in Food: Contamination.
55. Microbes Used in the Production of Foods.
56. Microbes in the Soil: The Nitrogen and Sulfur Cycles.
57. Microbes in Soil: Bioremediation.

APPENDICES.

A. Pipetting.
B. Dilution Techniques and Calculations.
C. Use of the Spectrophotometer.
D. Graphing.
E. Use of the Dissecting Microscope.
F. Use of the Membrane Filter.
G. Electrophoresis.
H. Keys to Bacteria.
Index.

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