ISBN-10:
0321644026
ISBN-13:
9780321644022
Pub. Date:
11/10/2010
Publisher:
Pearson
Laboratory Manual for Biotechnology and Laboratory Science: The Basics / Edition 1

Laboratory Manual for Biotechnology and Laboratory Science: The Basics / Edition 1

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Overview

Laboratory Manual for Biotechnology provides the basic laboratory skills and knowledge to pursue a career in biotechnology. The manual, written by four biotechnology instructors with over 20 years of teaching experience, incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities serve to engage and help you understand the fundamentals of working in a biotechnology laboratory. Building skills through an organized and systematic presentation of materials, procedures, and tasks, the manual will help you explore overarching themes that relate to all biotechnology workplaces.

The fundamentals in this manual are critical to the success of research scientists, scientists who develop ideas into practical products, laboratory analysts who analyze samples in forensic, clinical, quality control, environmental, and other testing laboratories.

Product Details

ISBN-13: 9780321644022
Publisher: Pearson
Publication date: 11/10/2010
Pages: 464
Product dimensions: 8.30(w) x 10.80(h) x 0.70(d)

About the Author

The authors of this manual are faculty in the Biotechnology Laboratory Technician Program at Madison Area Technical College, which prepares students to become laboratory professionals. These authors have years of experience and have spent a great deal of time in the classroom, trying out the exercises and activities which are laid out in the manual. Lisa Seidman is highly respected for her work in biotechnology instruction.

Table of Contents

INTRODUCTION

I. SAFETY IN THE LABORATORY

Unit Introduction

SAFETY PART 1: CREATING A SAFE WORKPLACE

Fundamental Principles

Classroom Activity 1: Performing a Risk Assessment

Classroom Activity 2: Exploring the CDC and OSHA Websites

Classroom Activity 3: Responding to Emergencies

SAFETY PART 2: WORKING SAFELY WITH CHEMICALS

Fundamental Principles

Classroom Activity 4: Understanding the Chemicals with which You Work

Classroom Activity 5: Personal Protection

Laboratory Exercise 1: Tracking the Spread of Chemical Contamination

Classroom Activity 6: Analyzing Safety Issues in a Laboratory Procedure

SAFETY PART 3: WORKING SAFELY WITH BIOLOGICAL HAZARDS

Fundamental Principles

Laboratory Exercise 2: Production of Bioaerosols and Factors Affecting Aerosol Production

Unit Discussion

Safety Contract, student copy

Safety Contract, to turn in

II. DOCUMENTATION IN THE LABORATORY

Unit Introduction

Classroom Activity 7: Be an Auditor

Classroom Activity 8: Writing and Following an SOP

Laboratory Exercise 3: Keeping a Laboratory Notebook

Unit Discussion

III. METROLOGY IN THE LABORATORY

Unit Introduction

Laboratory Exercise 4: Recording Measurements with the Correct Number of Significant Figures

Classroom Activity 9: Constructing a Simple Balance

Laboratory Exercise 5:Weight Measurements 1; Good Weighing Practices

Laboratory Exercise 6: Weight Measurements 2; Performance Verification

Laboratory Exercise 7: Volume Measurements 1; Proper Use of Volume Measuring Devices

Laboratory Exercise 8: Volume Measurements 2; Performance Verification of a Micropipette

Laboratory Exercise 9: Measuring pH with Accuracy and Precision

Unit Discussion

IV. SPECTROPHOTOMETRY

Unit Introduction

Laboratory Exercise 10: Color and the Absorbance of Light

Laboratory Exercise 11: Concentration, Absorbance, and Transmittance

Laboratory Exercise 12: Preparing a Standard Curve with Food Coloring and Using it for Quantitation

Classroom Activity 10:Beer’s Law and Calculating an Absorptivity Constant

Laboratory Exercise 13: Determination of the Absorptivity Constant for ONP

Unit Discussion

V. BIOLOGICAL SOLUTIONS

Unit Introduction

Classroom Activity 11: Getting Ready to Prepare Solutions with One Solute: Calculations Classroom Activity 12: Getting Ready to Prepare Solutions with One Solute: Ordering Chemicals

Laboratory Exercise 14: Preparing Solutions with One Solute

Laboratory Exercise 15: Preparing Solutions to the Correct Concentration

Laboratory Exercise 16: Working with Buffers

Laboratory Exercise 17: Preparing Breaking Buffer

Laboratory Exercise 18: Preparing TE Buffer

Laboratory Exercise 19: More Practice Making a Buffer

Laboratory Exercise 20: Making a Quality Product in a Simulated Company

Unit Discussion

VI. ASSAYS

Unit Introduction

Laboratory Exercise 21: Two Qualitative Assays

Laboratory Exercise 22:UV Spectrophotometry of DNA; Quantitative Applications

Laboratory Exercise 23:UV Spectrophotometry of DNA, RNA, and Proteins; Qualitative Applications

Laboratory Exercise 24: Using Spectrophotometry for Quality Control; Niacin

Laboratory Exercise 25: A Quantitative Protein Assay; Learning the Assay

Laboratory Exercise 26: Exploring Assay Verification

Laboratory Exercise 27: The Beta-Galactosidase Enzyme Assay

Laboratory Exercise 28: Comparing the Specific Activity of Two Preparations of Beta-Galactosidase

Unit Discussion

VII. SEPARATION METHODS

Unit Introduction

Classroom Activity 13: Separating Materials Using a Centrifuge, Planning

Laboratory Exercise 29: Separation of Two Substances Based on Their Differential Affinities for Two Phases

Laboratory Exercise 30: Separation of Dyes Using Paper Chromatography

Laboratory Exercise 31: Separating Molecules by Agarose Gel Electrophoresis

Laboratory Exercise 32: Using Agarose Gel Electrophoresis to Perform an Assay

Laboratory Exercise 33: Optimizing Agarose Gel Electrophoresis

Laboratory Exercise 34: Quantification of DNA by Agarose Gel Electrophoresis

Unit Discussion

VIII. GROWING CELLS

Unit Introduction

WORKING WITH BACTERIAL CELLS

Laboratory Exercise 35: Aseptic Technique on an Open Lab Bench

Laboratory Exercise 36: Working with Bacteria on an Agar Substrate; Isolating Individual Colonies

Laboratory Exercise 37: Using a Compound Light Microscope

Laboratory Exercise 38: Gram Staining

Laboratory Exercise 39: Making Phosphate Buffered Saline

Laboratory Exercise 40: The Aerobic Spread Plate Method of Enumerating Colony Forming Units

Laboratory Exercise 41: Generation of an E. coli Growth Curve

WORKING WITH MAMMALIAN CELLS

Laboratory Exercise 42: Aseptic Technique in a Laminar Flow Hood

Laboratory Exercise 43: Making Ham’s F12 Medium from Dehydrated Powder

Laboratory Exercise 44: Examining, Photographing, and Feeding CHO (Chinese Hamster Ovary) Cells

Laboratory Exercise 45: Counting Cells Using a Hemacytometer

Laboratory Exercise 46: Subculturing CHO Cells

Laboratory Exercise 47: Preparing a Growth Curve For CHO Cells

Unit Discussion

APPENDICES

I Materials and Supplies that are Assumed to be Present in Your Laboratory

II Calculating Standard Deviation

II Periodic Table of Elements

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