Laser Capture in Microscopy and Microdissection: Methods in Enzymology

Laser Capture in Microscopy and Microdissection: Methods in Enzymology

ISBN-10:
0121822591
ISBN-13:
9780121822590
Pub. Date:
10/21/2002
Publisher:
Elsevier Science

Hardcover

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Overview

Laser Capture in Microscopy and Microdissection: Methods in Enzymology

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.


  • Basic Principles, Specialized Uses, and Genetic Applications
  • LCM and its application in genomics and proteomics
  • Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections
  • Noncontact laser catapulting for the functional genomics and proteomics
  • Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more

Product Details

ISBN-13: 9780121822590
Publisher: Elsevier Science
Publication date: 10/21/2002
Pages: 775
Product dimensions: 8.08(w) x 7.86(h) x 0.22(d)

About the Author

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Table of Contents

Comparison of Current Equipment
Laser Capture Microdissection (LCMD) and its Applications in Genomics & Proteomics
Going in vivo With Laser MD (LMD)
Use of LCMD to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis
Optimized Tissue Processing and Staining for LCMD and Nucleic Acid Retrieval
Fluoroscence in situ Hybridization of LCM-isolated Nuclei from Paraffin Sections
Immunoblotting of Single Cell Types Isolated from Frozen Sections by LMD
Noncontact Laser Catapulting: A Basic Procedure for Functl' Genomics and Proteomics
Internal Standards for LMD
Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues
Use of LCMD for Clonal Analysis
LCMD in Carcinoma Analysis
LCMD to Assess Devt
Application of LCMD to Proteomics
LCMD of the Mouse Intestine: Characterizing mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified.
LCMD in Pathology
Use of Laser Capture Microscopy (LCM) in the Analysis of Mouse Models of Human Diseases
Use of LMD in Complex Tissue
Assessmt of Clonal Relationships in Malignant Melanomas
Comp. of Normal and Tumor Cells by LCMD
Analysis of Folliculostellate Cells by LCMD and Reverse Transcription-Polymerase Chain Reaction (LCM-RT/PCR)
Analysis of Gene Expression
Analysis of Specific Gene Expression
Gene Discovery With LCM
DNA Fingerprinting from Cells Captured by LMD
Single Cell PCR in LCM
Assessmt of Genetic Clonality in Tumors using LCMD
Gene Mutations: Analysis in Proliferative Prostatic Diseases Using LCMD
Use of LCMD Generated Targets for Hybridization of High-Density Oligonucleotide Arrays. Single Cell Gene Mutation Analysis Using Laser-Assisted MD of Tissue Sections
Methylation in Gene Promoters: Assessmt after LCMD

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