Life in the Frozen State

Life in the Frozen State

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Taylor & Francis
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Life in the Frozen State

While it is barely 50 years since the first reliable reports of the recovery of living cells frozen to cryogenic temperatures, there has been tremendous growth in the use of cryobiology in medicine, agriculture, horticulture, forestry, and the conservation of endangered or economically important species.

As the first major text on cryobiology in the genomic era, Life in the Frozen State describes the current understanding of how living cells and complex organisms survive very low temperatures. Leading world experts combine fundamental theory and practice across a spectrum of species and applications to evaluate how cryobiology can benefit humanity. Chapters encompass disciplines ranging from mathematical modeling and biophysics, to the molecular biology of stress gene expression and the clinical banking of cells and tissues.

This book provides a unique opportunity to explore the subject in a multidisciplinary context, which has historically been the key to realizing some of the most exciting advances in low temperature research.


  • Integrates fundamental theory and practice across a broad range of species and applications
  • Discusses cryobiology within a multidisciplinary context
  • Emphasizes how the current knowledge of cryobiology can be applied to benefit humanity through health care and conservation
  • Product Details

    ISBN-13: 9780415247009
    Publisher: Taylor & Francis
    Publication date: 05/01/2004
    Edition description: New Edition
    Pages: 704
    Product dimensions: 6.75(w) x 9.75(h) x 1.56(d)

    Table of Contents

    Principles of Cryobiology, Peter Mazur
    The Water to Ice Transition - Implications for Living Cells, Ken Muldrew, Jason Acker, Gloria Elliott, and Locksley McGann

    Life in the Polar Terrestrial Environment with a Focus on Algae and Cyanobacteria, Josef Elster and Erica Benson
    Microbial Life in Permafrost: Extended Time in Extreme Conditions, Monica Ponder, Tatiana Vishnivetskaya, John McGrath, and James Tiedje
    Adaptation of Higher Plants to Freezing, Roger Pearce
    Oxidative Stress in the Frozen Plant: A Free Radical Point of View, Erica Benson and David Bremmer
    Physiology, Biochemistry, and Molecular Biology of Vertebrate Freeze Tolerance: The Wood Frog, Kenneth B. Storey and Janet M. Storey

    Preservation of Fungi and Yeasts, C. Shuhui Tan and Cor van Ingen
    Cryo-conserving Algal and Plant Diversity: Historical Perspectives and Future Challenges, Erica Benson
    Plant Cryopreservation, Akira Sakai
    The Early History of Gamete Cryobiology, Stanley P. Leibo
    Cryobiology of Gametes and the Breeding of Domestic Animals, Alban Massip, Stanley P. Leibo, and Elizabeth Blesbois
    Cryopreservation as a Supporting Measure in Species Conservation: "Not the Frozen Zoo", Amanda R. Pickford and William V. Holt
    Cryopreservation of Gametes and Embryos of Aquatic Species, Tiantian Zhang
    Fundamental Issues for Cell-line Banks in Biotechnology and Regulatory Affairs, Glyn Stacey

    Mechanisms of Injury Caused by in vivo Freezing, Nathan E. Hoffman and John C. Bischof
    Cryopreservation in Transfusion Medicine and Haematology, Andreas Sputtek and Rebekka Sputtek
    Cryopreservation of Human Gametes and Embryos, Barry Fuller, Sharon Paynter, and Paul Watson
    The Scientific Basis for Tissue Banking, Monica Wusteman and Charles J. Hunt
    Engineering Desiccation Tolerance in Mammalian Cells: Tools and Techniques, Jason P. Acker, Tani Chen, Alex Fowler, and Mehmet Toner
    Stabilization of Cells During Freeze-Drying: The Trehalose Myth, John H. Crowe, Lois M. Crowe, Fern Tablin, William Wolkers, Ann E. Oliver, and Nelly M. Tsvetkova
    Vitrification in Tissue Preservation: New Developments, Mike J. Taylor, Yin C. Song, and Kelvin G.M. Brockband

    The Future of Cryobiology, Nick Lane, Barry Fuller, and Erica Benson

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