Liposomes: Methods and Protocols, Volume 1: Pharmaceutical Nanocarriers

Liposomes: Methods and Protocols, Volume 1: Pharmaceutical Nanocarriers

by Volkmar Weissig (Editor)


Choose Expedited Shipping at checkout for guaranteed delivery by Monday, July 22


With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposome: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of all cell membrane. Volume 1: Pharmaceutical Nanocarriers focuses on the state-of-the-art aspects of developing liposome-based nanoscale drug delivery techniques, delving into the depths of the emerging field of pharmaceutical and biomedical nanotechnology. As a volume in the highly successful Methods in Molecular Biology? series, the chapters include introductions to their respective topics, life of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Liposomes: Methods and Protocols promise to be an essential source of practical know-how for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol.

Product Details

ISBN-13: 9781603273596
Publisher: Springer-Verlag New York, LLC
Publication date: 12/18/2009
Series: Methods in Molecular Biology , #605
Edition description: 2010
Pages: 564
Product dimensions: 7.00(w) x 10.10(h) x 1.50(d)

Table of Contents

Preface v

Contributors xi

1 Current Trends in Liposome Research Tamer A. ElBayoumi Vladimir P. Torchilin 1

2 Nanoliposomes: Preparation and Analysis M.R.Mozafari 29

3 Preparation of DRV Liposomes Sophia G. Antimisiaris 51

4 Elastic Liposomes for Topical and Transdermal Drug Delivery Heather A.E. Benson 77

5 Archaebacterial Tetraetherlipid Liposomes Aybike Ozcetin Samet Mutlu Udo Bakowsky 87

6 Cationic Magnetoliposomes Marcel De Cuyper Stefaan J.H. Soenen 97

7 Ultrasound-Responsive Liposomes Shao-Ling Huang 113

8 Liposome Formulations of Hydrophobic Drugs Reto A. Schwendener Herbert Schott 129

9 Remote Loading of Antbracyclines into Liposomes Felicitas Lewrick Regine Peschka Süss 139

10 Arsonoliposomes: Preparation and Physicochemical Characterization Sophia G. Antimisiaris Panayiotis V. Ioannou 147

11 Liposome-Based Vaccines Reto A. Schwendener Burkhard Ludewig Andreas Cerny Olivier Engler 163

12 Mannosylated Liposomes for Targeted Vaccines Delivery Suresh Prasad Vyas Amit K. Goyal Kapil Khatri 177

13 Liposomes for Specific Depletion of Macrophages from Organs and Tissues Nico van Rooijen Esther Hendrikx 189

14 Vesicular Phospholipid Gels Martin Brandl 205

15 Environment-Responsive Multifunctional Liposomes Amit A. Kale Vladimir P. Torchilin 213

16 Functional Liposomal Membranes for Triggered Release Armağan Koçer 243

17 A "Dock and Lock" Approach to Preparation of Targeted Liposomes Marina V. Backer Joseph M. Backer 257

18 Conjugation of Ligands to the Surface of Preformed Liposomes by Click Chemistry Benoît Frisch Fatouma Saïd Hassane Francis Schuber 267

19 Targeted Magnetic Liposomes Loaded with Doxorubicin Pallab Pradhan Rinti Banerjee Dhirendra Bahadur Christina Koch Olga Mykbaylyk Christian Plank 279

20 Liposomes for Drug Delivery to Mitochondria Sarathi V. Boddapati Gerard G.M. D'Souza Volkmar Weissig 295

21 Cytoskeletal-Antigen Specific Immunoliposomes: Preservation of Myocardial Viability Vishwesh Patil Tala Khudairi Ban-An Khaw 305

22 Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes Suna Erdogan Vladimir P. Torchilin 321

23 Angiogenic Vessel-Targeting DDS by Liposomalized Oligopeptides Tomohiro Asai Naoto Oku 335

24 TAT-Peptide Modified Liposomes: Preparation, Characterization, and Cellular Interaction Marjan M. Fretz Gert Storm 349

25 ATP-Loaded Liposomes for Targeted Treatment in Models of Myocardial Ischemia Tatyana S. Levchenko William C. Hartner Daya D. Verma Eugene A. Bernstein Vladimir P. Torchilin 361

26 Intracellular ATP Delivery Using Highly Fusogenic Liposomes Sufan Chien 377

27 Lipoplex Formation Using Liposomes Prepared by Ethanol Injection Yoshie Maitani 393

28 Acid-Labile Liposome/pDNA Complexes Michel Bessodes Daniel Scherman 405

29 Serum-Resistant Lipoplexes in the Presence of Asialofetuin Conchita Tros de ILarduya 425

30 Anionic pH Sensitive Lipoplexes Nathalie Mignet Daniel Scherman 435

31 Liposomal siRNA Delivery Jeffrey Hughes Preeti Yadava Ryan Mesaros 445

32 Complexation of siRNA and pDNA with Cationic Liposomes: The Important Aspects in Lipoplex Preparation José Mario Barichello Tatsuhiro Ishida Hirosht Kiwada 461

33 Effective In Vitro and In Vivo Gene Delivery by the Combination of Liposomal Bubbles (Bubble Liposomes) and Ultrasound Exposure Ryo Suzuki Kazuo Maruyama 473

34 Liposomal Magnetofection Olga Mykbaylyk Yolanda Sánchez Antequera Dialekti Vlaskou Edelburga Hammerschmid Martina Anton Olivier Zelphati Christian Plank 487

35 Long-Circulating, pH-Sensitive Liposomes Denitsa Momekova Stanislav Rangelov Nikolay Lambov 527

36 Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes Nicolas Bertrand Pierre Simard Jean-Christophe Leroux 545

Index 559

Customer Reviews

Most Helpful Customer Reviews

See All Customer Reviews