Listeria monocytogenes: Methods and Protocols

Listeria monocytogenes: Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2014)

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Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series, Listeria monocytogenes: Methods and Prools addresses its titular pathogen with prools and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation and finally, methods for control of the organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools and tips on troubleshooting and avoiding known pitfalls.

Vital and authoritative, Listeria monocytogenes: Methods and Prools aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research.

Product Details

ISBN-13: 9781493954360
Publisher: Springer New York
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #1157
Edition description: Softcover reprint of the original 1st ed. 2014
Pages: 286
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Part I: Detection, Quantification, and Confirmation

1. Sampling the Production/Processing Environment for Listeria

Anca Ioana Nicolau and Andrei Sorin Bolocan

2. Traditional Methods for Isolation of Listeria monocytogenes

Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, and Paula Teixeira

3. Confirmation of Isolates of Listeria by Conventional and Real-Time PCR

David Rodríguez-Lázaro and Marta Hernández

Part II: Characterization and Typing

4. Serotype Assignment by Sero-Agglutination, ELISA, and PCR

Lisa Gorski

5. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes

Marion Dalmasso and Kieran Jordan

6. Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes

Beatrix Stessl, Irene Rückerl, and Martin Wagner

7. Ribotyping and Automated Ribotyping of Listeria monocytogenes

Mazin Matloob and Mansel Griffiths

8. Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes

Corinne Amar

9. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray

Edward M. Fox and Kieran Jordan

10. Analysis of Listeria monocytogenes Subproteomes

Michel Hébraud

11. The Listeria Cell Wall and Associated Carbohydrate Polymers

Marcel R. Eugster and Martin J. Loessner

12. Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria

Mathias Schmelcher and Martin J. Loessner

13. Virulence Characterization of Listeria monocytogenes

Swetha Reddy and Mark L. Lawrence

14. Internalization Assays for Listeria monocytogenes

Andreas Kühbacher, Pascale Cossart, and Javier Pizarro-Cerdá

Part III: Strain Manipulation

15. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes

Aidan Casey and Olivia McAuliffe

16. Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method

Kathrin Rychli, Caitriona M. Guinane, Karen Daly, Colin Hill, and Paul D. Cotter

17. Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes

Reha Onur Azizoglu, Driss Elhanafi, and Sophia Kathariou

18. Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR

Roberta Mazza and Rina Mazette

19. Genome Sequencing of Listeria monocytogenes

Stephan Schmitz-Esser and Martin Wagner

20. Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels

Marta Utratna and Conor P. O’Byrne

Part IV: Control Methods

21. Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers

Stavros G. Manios and Panagiotis N. Skandamis

22. Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique

Mohammed Bahey-El-Din and Cormac G.M. Gahan

23. Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments

Martin Wagner and Beatrix Stessl

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