Pub. Date:
Springer-Verlag New York, LLC
Live Cell Imaging: Methods and Protocols / Edition 1

Live Cell Imaging: Methods and Protocols / Edition 1

by Dmitri PapkovskyDmitri Papkovsky


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Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine. Written for: Molecular and cellular biologists, chemists, physicists, optics specialists, engineers, cell physiologists, and medical doctors

Product Details

ISBN-13: 9781607614036
Publisher: Springer-Verlag New York, LLC
Publication date: 12/17/2009
Series: Methods in Molecular Biology , #591
Edition description: 2010
Pages: 367
Product dimensions: 7.20(w) x 10.10(h) x 1.20(d)

Table of Contents

Preface v

Contributors ix

Part I General Principles and Overview 1

1 Instrumentation for Live-Cell Imaging and Main Formats Martin Oheim 3

2 Labels and Probes for Live Cell Imaging: Overview and Selection Guide Scott A. Hilderbrand 17

3 Live Cell Imaging: An Industrial Perspective Terry McCann 47

Part II Imaging Techniques, Probes, and Applications 67

4 Design of Fluorescent Fusion Protein Probes Elizabeth Pham Kevin Truong 69

5 Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cells Dan Yang Zhen-Ning Sun Tao Peng Hua-Li Wang Jian-Gang Sben Yan Chen Paul Kwong-Hang Tarn 93

6 Photo-Activatable Probes for the Analysis of Receptor Function in Living Cells Wen-Hong Li 105

7 The Application of Fluorescent Probes for the Analysis of Lipid Dynamics During Phagocytosis Ronald S. Flannagan Sergio Grinstein 121

8 Imaging of Mitotic Cell Division and Apoptotic Intra-Nuclear Processes in Multicolor Kenji Sugimoto Shigenobu Tone 135

9 Manipulation of Neutrophil-Like HL-60 Cells for the Study of Directed Ceil Migration Arthur Millius Orion D. Weiner 147

10 A Method for Analyzing Protein-Protein Interactions in the Plasma Membrane of Live B Cells by Fluorescence Resonance Energy Transfer Imaging as Acquired by Total Internal Reflection Fluorescence Microscopy Hae Won Sohn Pavel Tolar Joseph Brzostowski Susan K. Pierce 159

11 Sample Preparation for STED Microscopy Christian A. Wurm Daniel Neumann Roman Schmidt Alexander Egner Stefan Jakobs 185

12 Two-Photon Permeabilization and Calcium Measurements in Cellular Organelles Oleg Gerasimenko Julia Gerasimenko 201

13 Imaging and Analysis of Three-Dimensional Cell Culture Models Benedikt W. Graf Stephen A. Boppart 211

14 Long-Term Imaging in Microfluidic Devices Gilles Charvin Catherine Oikonomou Frederick Cross 229

15 Monitoring of Cellular Responses to Hypoxia Christoph Wotzlaw Joachim Fandrey 243

16 Imaging of Cellular Oxygen and Analysis of Metabolic Responses of Mammalian Cells Andreas Fercher Tomas C. O'Riordan Alexander V. Zhdanov Ruslan I. Dmitriev Dmitri B. Papkovsky 257

17 Analysis of Mitochondrial pH and Ion Concentrations Martin vandeVen Corina Balut Szilvia Baron Ilse Smets Paul Steels Marcel Ameloot 275

18 Live Cell Imaging Analysis of Receptor Function Daniel C. Worth Maddy Parsons 311

19 Subcellular Dynamic Imaging of Protein-Protein Interactions in Live Cells by Biohiminescence Resonance Energy Transfer Julie Perroy 325

20 Quantitative Analysis of Membrane Potentials Mmius W. Ward 335

21 Image Correlation Spectroscopy to Define Membrane Dynamics Jeremy Bonor Anja Nohe 353

Subject Index 365

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