ISBN-10:
9814307343
ISBN-13:
9789814307345
Pub. Date:
09/01/2010
Publisher:
World Scientific Publishing Company, Incorporated
Live Imaging In Zebrafish: Insights Into Development And Disease

Live Imaging In Zebrafish: Insights Into Development And Disease

by Karuna Sampath, Sudipto Roy

Hardcover

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Product Details

ISBN-13: 9789814307345
Publisher: World Scientific Publishing Company, Incorporated
Publication date: 09/01/2010
Pages: 164
Product dimensions: 6.00(w) x 9.00(h) x 0.60(d)

Table of Contents

Preface ix

Chapter 1 Analysis of Branchiomotor Neuron Migration in the Zebrafish Petra Stockinger Carl-Philipp Heisenberg 1

Introduction 2

Imaging of FBMN Migration In Vivo 5

Outlook 11

Acknowledgments 13

References 13

Chapter 2 Imaging the Nervous System: Insights Into Central and Peripheral Glia Development Sarah Kucenas Bruce Appel 17

Introduction 18

Central and Peripheral Nervous System Development 18

In Vivo Imaging 20

Central Nervous System 21

Peripheral Nervous System 25

Conclusions 30

References 31

Chapter 3 Imaging the Cell Biology of Neuronal Migration in Zebrafish Martin Distel Jennifer Hocking Reinhard W. Köster 35

Introduction 36

Neuronal Migration 37

Advances in Technology 43

Cell Biology of Neuronal Migration: More Questions Than Answers 55

Conclusions 62

Acknowledgments 63

References 63

Chapter 4 Applications of Fluorescence Correlation Spectroscopy in Living Zebrafish Embryos Xianke Shi Yong Hwee Foo Vladimir Korzh Sohail Ahmed Thorsten Wohland 69

Introduction 70

Introduction to FCS 72

Application of FCS in Zebrafish Embryos 83

Conclusion 95

References 96

Chapter 5 Real-Time Imaging of Lipid Metabolism in Larval Zebrafish Juliana D. Carten Steven A. Farber 105

Introduction 106

The Clinical Importance of Lipid Therapeutics 106

Limitations of In Vitro Lipid Metabolism Studies 107

Zebrafish as Models of Human Physiology and Disease 108

Lipid Metabolism is Conserved in Zebrafish 108

Dietary Lipid Metabolism in Zebrafish 109

Real-Time Imaging of Intestinal Lipid Metabolism: Fluorescent Reporters 112

Fluorescent Microspheres 114

Triple Screening: PED6, EnzChek, and Microspheres 115

Concluding Thoughts 119

Acknowledgments 120

Financial Disclosure 121

References 121

Chapter 6 Live Imaging Innate Immune Cell Behavior During Normal Development, Wound Healing and Infection Chris Hall Maria Vega Flores Makoto Kamei Kathryn Crosier Phil Crosier 129

Introduction 130

Mounting Strategies for Live Imaging 132

Investigating the Physiological Behaviors of Myeloid Leukocytes During Normal Development 134

Live Imaging the Response of Myeloid Leukocytes to Wounding 137

Live Imaging the Leukocytic Response to Bacterial Infection 142

Acknowledgments 144

References 144

Index 149

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