Mammalian Synthetic Promoters

Mammalian Synthetic Promoters

by David Gould (Editor)

Hardcover(1st ed. 2017)

$169.99
Use Standard Shipping. For guaranteed delivery by December 24, use Express or Expedited Shipping.

Product Details

ISBN-13: 9781493972210
Publisher: Springer New York
Publication date: 08/12/2017
Series: Methods in Molecular Biology , #1651
Edition description: 1st ed. 2017
Pages: 277
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

1. Initial Considerations before Designing a Promoter Construct

David Gould



Part I: Monitoring Promoter Interactions and Activity


2 Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay


Nasim Yousaf and David Gould



3. Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter


Jordan Read



4. Secreted Reporters for Monitoring Multiple Promoter Function


Ghazal Lashgari, Rami S. Kantar, and Bakhos A. Tannous



5. Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo


Juliette MKM Delhove, Rajvinder Karda, Kate E Hawkins, Simon N Waddington, and Tristan R McKay



6. Monitoring Promoter Activity by Flow Cytometry


Taher E. I. Taher



Part II: Promoter Design and Applications


7. Functional Screening of Core Promoter Activity


Dan Y. Even, Adi Kedmi, Diana Ideses, and Tamar Juven-Gershon



8. Bioinformatically Informed Design of Synthetic Mammalian Promoters


Michael L. Roberts, Polyxeni Katsoupi, Vivian Tsevleki, and Era Taoufik



9. Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication


M. Veronica Lopez, Eduardo. G. Cafferata, Diego. L. Viale, and Osvaldo Podhajcer



10. Constructing Strong Cell-Type-Specific Promoters through Informed Design


Adam J. Brown and David C. James



11. PCR Assembly of Synthetic Promoters



Hodan Mohamed and David Gould



Part III: Regulated Promoter Approaches


12. The Tetracycline Responsive System



Nasim Yousaf and David Gould



13. Light-responsive Promoters


Maximilian Hörner, Konrad Müller, and Wilfried Weber



14. A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation


Ryohei Ogawa, Go Kagiya, Akihiko Watanabe, Akihiro Morii, Zheng-Guo Cui, and Takashi Kondo


15. Promoter Activation with Electromagnetism


Abraham O. Rodríguez-De la Fuente, J. Antonio Heredia-Rojas, Juan M. Alcocer-González, Laura E. Rodríguez-Flores, Cristina Rodríguez-Padilla, and Reyes S. Taméz-Guerra



16. Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment


Eduardo G Cafferata, M Verónica López, Felipe J Nuñez, María Agostina Russo Maenza, and Osvaldo L Podhajcer



Part IV: Combining Promoters in Synthetic Circuits


17. A Modular Approach to Building Complex Synthetic Circuits


Yinqing Li and Ron Weiss



18. Computational Sequence Design with R2oDNA Designer



James T. MacDonald and Velia Siciliano



19. Design of Synthetic Promoters for Gene Circuits in Mammalian Cells


Pratik Saxena, Daniel Bojar, and Martin Fussenegger

Customer Reviews

Most Helpful Customer Reviews

See All Customer Reviews