This volume aims to outline the current status of the Mesenchymal Stem Cells(MSC) field in regenerative medicine and to propose clear and reproducible protocols to better define the identity, function and use of these cells that are today, more than ever, “under the spotlight”. Mesenchymal Stem Cells: Methods and Protocols, Second Edition is organized into four sections. The first guides the reader through a series of state-of-the-art reviews summarizing the use of MSC for the treatment of various diseases. The other three sections are a collection of methodological chapters covering several aspects: isolation and characterization of MSC; expansion of MSC for clinical use; production and characterization of the MSC secretome. Written in the highly successful Methods in Molecular Biology series format, the method chapters include introductions to their respective topics, complete lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting which will help the researcher to avoid known pitfalls.
Authoritative and cutting-edge, Mesenchymal Stem Cells: Methods and Protocols, Second Edition, aims to ensure successful results in the further study of this vital field.
Table of Contents
Mesenchymal Stromal Cells In Hematopoietic Stem Cell Transplantation.- Bone Tissue Engineering: Past – Present – Future.- Mesenchymal Stem Cells For Osteochondral Tissue Engineering.- Mesenchymal Stem Cells In Cardiology.- Mesenchymal Stem Cells In Kidney Repair.- Mesenchymal Stem Cells In Lipogems, A Reverse Story: From Clinical Practice To Basic Science.- Paracrine Mechanisms Of Mesenchymal Stem Cells In Tissue Repair.- Protocols For In Vitro Differentiation Of Human Mesenchymal Stem Cells Into Osteogenic, Chondrogenic And Adipogenic Lineages.- Colony Forming Unit Assays.- Methods and Strategies For Lineage Tracing Of Mesenchymal Progenitor Cells.- Isolation Of Mouse Bone Marrow Mesenchymal Stem Cells.- Isolation Of Pig Bone Marrow-Derived Mesenchymal Stem Cells.- Isolation, Culture, And Phenotypic Characterization Of Mesenchymal Stromal Cells From The Amniotic Membrane Of Human Term Placenta.- Isolation, Culture And Characterization Of Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells.- Isolation, Expansion And Immortalization Of Human Adipose-Derived Mesenchymal Stromal Cells From Biopsies And Liposuction Specimens.- Optimization Of Mesenchymal Stem Cells To Increase Their Therapeutic Potential.- Directed Differentiation Of Human Induced Pluripotent Stem Cells To Mesenchymal Stem Cells .- Isolation And Manufacture Of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells.- Quality Control Assays For Clinical Grade Human Mesenchymal Stromal Cells: Methods For ATMP Release.- Quality Control Assays For Clinical Grade Human Mesenchymal Stromal Cells: Validation Strategy.- Cryopreservation And Revival Of Human Mesenchymal Stromal Cells.- Clinical-Grade Manufacturing Of Therapeutic Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Culture Systems.- GMP-Compliant Expansion of Clinical Grade Human Mesenchymal Stromal/Stem Cells Using a Closed Hollow-Fibre Bioreacto.- Engineering Small-Scale and Scaffold-Based Bone Organs Via Endochondral Ossification Using Adult Progenitor Cells.- Fabrication Of Elasticity-Tunable Gelatinous Gel For Mesenchymal Stem Cell Culture.- Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium.- Tips on How to Collect and Administer the Mesenchymal Stem Cell Secretome for Central Nervous System Applications.- Soluble Factors from Human Fetal Bone Marrow-Derived Mesenchymal Stem Cells: Preparation of Conditioned Medium and its Effect On Tumor Cells.- Isolation and Characterization of Exosome From Human Embryonic Stem Cell-Derived Cmyc-Immortalized Mesenchymal Stem Cells.- Transcriptomic Analysis of Adult Renal Derived Mesenchymal Stem-Like Cells.- Proteomic Analysis of Mesenchymal Stem Cells.- Unraveling Mesenchymal Stem Cells’s Dynamic Secretome Through Non-Targeted Proteomics.- Identification Of Factors Produced And Secreted By Mesenchymal Stromal Cells With The SILAC Method.