ISBN-10:
1493948873
ISBN-13:
9781493948871
Pub. Date:
08/27/2016
Publisher:
Springer New York
Metastasis Research Protocols / Edition 2

Metastasis Research Protocols / Edition 2

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Overview

Cancer specialists from a wide range of disciplines seek to encompass the entire spectrum of cancer biology in the two volumes that can serve as stand-along references for both new and experienced researchers. Volume two focuses sharply on determining cell behavior, combining established and familiar techniques such as SDS-PAGE and Western blotting with newer and more specialized approaches and specific examples of their application. The general themes are selecting and segregating cell populations for in-vitro and in-vivo assays of metastatic behavior, in-vitro assays of metastatic behavior, and animal models.

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Product Details

ISBN-13: 9781493948871
Publisher: Springer New York
Publication date: 08/27/2016
Series: Methods in Molecular Biology , #1070
Edition description: Softcover reprint of the original 2nd ed. 2014
Pages: 250
Product dimensions: 7.01(w) x 10.00(h) x 0.02(d)

Table of Contents

1. Basic Immunocytochemistry for Light Microscopy

Susan A. Brooks

2. Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues

Susan A. Brooks & Debbie M.S. Hall

3. Histopathological Assessment of Metastasis

Derek E Roskell and Ian D Buley

4. Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells

Stephan Braun and Klaus Pantel

5. The Polymerase Chain Reaction (PCR)

Hazel M. Welch

6. SDS-PAGE and Western Blotting Techniques

Christine Blancher and Rob Mc Cormick

7. 2-DE Based Proteomics for the Analysis of Metastasis Associated Proteins

Miriam V Dwek and Diluka Peiris

8. Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography

Marta Toth, Anjum Sohail, and Rafael Fridman

9. Determination of Cell Specific Receptor Binding using a Combination of Immunohistochemistry and in vitro Autoradiography: Relevance to Therapeutic Receptor Targeting in Cancer

Michael R. Dashwood and Marilena Loizidou

10. Fluorescence in situ Hybridization (FISH) for Cancer Related Studies

Lyndal Kearney and Janet Shipley

11. Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells

Julien Saint Guirons and Björn Ingemarsson

12. Cell Separations by Flow Cytometry

Derek Davies

13. Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cells Cultures

Matthias Christgen, Matthias Ballmaier, Ulrich Lehmann and HansKreipe

14. Genes Involved in the Metastatic Cascade of Medullary Thyroid Tumours

Caroline Schreiber, Kirsten Vormbrockand Ulrike Ziebold

15. High-resolution Quantitative Methylation Analysis of microRNA Genes using Pyrosequencing™

Ulrich Lehmann, Cord Albat, Hans Kreipe

16. RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive Example

Daniel Wicklein

17. Galectin-3-Binding and Metastasis

Pratima Nangia-Makker, Vitaly Balan, and Avraham Raz

18. Lectin Array Based Strategies for Identifying Metastasis-associated Changes in Glycosylation

Simon Fry, Babak Afrough, Anthony Leathem and Miriam V Dwek

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