Pub. Date:
Springer-Verlag New York, LLC
Metastasis Research Protocols / Edition 1

Metastasis Research Protocols / Edition 1

by Susan A. Brooks, Udo Schumacher
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Product Details

ISBN-13: 9781617370991
Publisher: Springer-Verlag New York, LLC
Publication date: 11/09/2010
Series: Methods in Molecular Medicine , #58
Edition description: Softcover reprint of hardcover 1st ed. 2001
Pages: 310
Product dimensions: 5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Part I. Selection/Segregation of Cell Populations for In vitro and In Vivo Assays of Metastatic Behavior

Cell Separations by Flow Cytometry
Derek Davies

Immunomagnetic Cell Separation
Catherine Clarke and Susan Davies

Genetic Modification of Cell Lines to Enhance Their Metastatic Capability
Daniel McWilliams and Hilary Collins

Part II. In Vitro Assays of Metastatic Behavior

Cell Aggregation Assays, Tom Boterberg
Marc E. Bracke, Erik A. Bruyneel, and Marc M. Mareel

Cell Migration and the Boyden Chamber
Nicholas S. Brown and Roy Bicknell

Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay
Yasufumi Niinaka, Arayo Haga, and Avraham Raz

In Vitro Invasion Assay Using Matrigel®
Debbie M. S. Hall and Susan A. Brooks

Membrane Invastion Culture System
Mary J. C. Hendrix, Elisabeth A. Seftor, and Richard E. B. Seftor

Collagen Invasion Assay
Marc E. Bracke, Tom Boterberg, Erik A. Bruyneel, and Marc M. Mareel

Chick Heart Invasion Assay
Marc E. Bracke, Tom Boterberg, and Marc M. Mareel

Adhesion of Tumor Cells to Matrices and Endothelium
G. Ed Rainger

Assessment of Angiogenic Factors: The Chick Chorioallantoic Membrane Assay
Adam Jones, Chisato Fujiyama, Stephen Hague, and Roy Bicknell

Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis
Nerbil Kilic and Süleyman Ergün

Galectin-3 Binding and Metastasis
Pratima Nangia-Makker, Yuichiro Honjo, and Avraham Raz

Part III. Animal Models of Metastasis

Basic Principles for the Study of Metastasis Using Animal Models
Suzanne A. Eccles

The Chick Embryo Metastasis Model
Eric Petitclerc and Peter C. Brooks

Gene Transfection for Metastasis ResearchUsing Animal Models
Ruth J. Muschel and Jim Hua

Theoretical Considerations in Using Animal Models of Metastastis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice
Sue A. Watson and Teresa M. Morris

Xenograft Models in Immunodeficient Animals: I. Nude Mice: Spontaneous and Experimental Metastatis Models
Janet E. Price

Xenograft Models in Immunodeficient Animals: II. Use of SCID Mice in Metastasis Research: Breast and Colon Cancer Models of Metastasis
Udo Schumacher and Susan A. Brooks

Syngeneic Murine Metastasis Model: B16 Melanoma
Raffaella Giavazzi and Angela Garofalo

Transgenic Animal Models
Chantale Guy and Gizela Cordosa

Clinically Accurate Orthotopic Mouse Models of Cancer
Robert M. Hoffman

Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo
Marian Rocha, Volker Schirrmacher, and Victor Umansky

Green Fluorescent Protein for Metastastis Research
Robert M. Hoffman

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