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Methods in Biological Oxidative Stress
     

Methods in Biological Oxidative Stress

by Kenneth Hensley (Editor)
 

Methods in Biological Oxidative Stress is practical in scope, and outlines modern techniques for measuring oxidative stress and detecting oxidizing agents both in vitro and in vivo. Free radicals and reactive oxidizing agents, once ignored as meaningful biochemicals entities, are now recognized as causes or contributing factors in dozens if not hundreds of disease

Overview

Methods in Biological Oxidative Stress is practical in scope, and outlines modern techniques for measuring oxidative stress and detecting oxidizing agents both in vitro and in vivo. Free radicals and reactive oxidizing agents, once ignored as meaningful biochemicals entities, are now recognized as causes or contributing factors in dozens if not hundreds of disease states, and free radical metabolism of xenobiotics is increasingly important to pharmacologists. This is the first book to detail experimental methods for detecting free radicals and reactive oxygen species or their byproducts. Subjects covered include techniques used routinely in laboratories developed by the authors, as well as HPLC-electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine; HPLC-electrochemical detection of DNA oxidation products; HPLC measurement of salicylate products, a means of detecting OH radicals in vivo; electron paramagnetic resonance. Contributions are from recognized experts in the field of oxidative stress, who have developed novel strategies for studying biological oxidations so that clinical laboratories and teaching institutions will find this release invaluable as a manual to assist in conducting routine measurements of biological oxidants and biological oxidative stress making it a reference work which will remain in demand for some years to come.

Product Details

ISBN-13:
9780896038158
Publisher:
Springer-Verlag New York, LLC
Publication date:
02/15/2010
Series:
Methods in Pharmacology and Toxicology Series
Edition description:
2003
Pages:
216
Product dimensions:
6.10(w) x 9.25(h) x 0.02(d)

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