Methods in Membrane Biology

Methods in Membrane Biology

by Edward D. Korn

Paperback(1976)

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Product Details

ISBN-13: 9781475758221
Publisher: Springer US
Publication date: 07/31/2013
Edition description: 1976
Pages: 267
Product dimensions: 5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

1 Preparation and Use of Liposomes as Models of Biological Membranes.- 1. Introduction and Historical Survey.- 2. Model Systems.- 2.1. Multilamellar Liposome.- 2.2. Microvesicle.- 2.3. Macrovesicles.- 3. Physical Properties of Membrane Molecules in Aqueous Media.- 3.1. Microscopy.- 3.2. X-Ray Diffraction.- 3.3. Light Scattering.- 3.4. Microcalorimetry.- 3.5. Centrifugation.- 3.6. Temperature Jump.- 3.7. Nuclear Magnetic Resonance.- 3.8. Spin Labels.- 3.9. Microelectrophoresis.- 4. Methods.- 4.1. Surface Area.- 4.2. Dialysis.- 4.3. Equilibrium Diffusion (Low Permeabilities).- 4.4. Nonequilibrium Fluxes (High Permeabilities).- 5. Materials.- 5.1. Egg Phosphatidylcholine.- 5.2. Phosphatidic Acid.- 5.3. Ox-Brain Phospholipids.- 5.4. Characterization and Purity.- 6. References.- 2 Thermodynamics and Experimental Methods for Equilibrium Studies with Lipid Monolayers.- 1. Introduction.- 2. Thermodynamics.- 2.1. The Monolayer System.- 2.2. Equilibrium and Supercompressed States.- 2.3. The Gibbs Phase Rule and Phase Separation in Monolayers.- 2.4. The Film Balance Experiment: A Thermodynamic Analysis.- 2.5. Molecular Thermodynamics of Lipid Monolayers.- 3. Experimental Methods.- 3.1. The Film Balance.- 3.2. Evaluation of ? Using Radiotracers.- 3.3. Preparation and Use of Materials for the Film Balance.- 3.4. Other Techniques.- 4. References.- 3 Circular Dichroism and Absorption Studies on Biomembranes.- 1. Introduction.- 1.1. On the Complexity of Biomembrane Spectra.- 2. Distortions in the Absorption and Circular Dichroism Spectra of Biomembranes.- 2.1. Absorption Effects.- 2.2. Circular Dichroism: Differential Absorption Effects.- 2.3. Instrumental Artifacts.- 3. On Calculations of the Particulate Poly-L-Glutamic Acid Model System.- 4. On the Presence or Absence of Artifact at 222 nm in the CD of Biomembranes.- 5. Approximate Corrections.- 5.1. Pseudo Reference State Approach.- 5.2. Consideration of Membrane Heterogeneity.- 5.3. On Alternate Sources of Absorption Data.- 6. Analysis of Corrected Membrane Data.- 6.1. Specialized Structures Proposed for Specialized Functions.- 6.2. Domains.- 6.3. Empirical Correlations.- 7. References.- 4 Isolation and Serological Evaluation of HL-A Antigens Solubilized from Cultured Human Lymphoid Cells.- 1. Introduction.- 2. Extraction of Soluble Histocompatibility Antigens.- 2.1. Cell Surface Location.- 2.2. Criteria of Solubility.- 2.3. Antigen Source Material.- 2.4. Extraction Techniques.- 2.5. Mechanism of Action of Chaotropic Ions.- 3. Purification of HL-A Antigens.- 3.1. Preparative PAGE.- 4. Physicochemical Characterization of HL-A Antigens.- 4.1. Physical Properties.- 4.2. Chemical Properties.- 5. Molecular Representation of HL-A Antigens on the Lymphoid Cell Surface.- 5.1. Membrane-Antigen Models.- 6. Serological Assays.- 6.1. The Cytotoxic Test.- 6.2. The Blocking Assay.- 6.3. The Quantitative Absorption Test.- 6.4. Comments and Conclusions.- 7. Perspectives.- 8. References.- 5 Dissociation and Reassembly of the Inner Mitochondrial Membrane.- 1. Components of the Inner Mitochondrial Membrane.- 1.1. Introduction.- 1.2. Electron Transport System.- 1.3. Energy Transfer System.- 1.4. Ion Transport and Membrane Lipids.- 1.5. Topology in the Membrane: Extrinsic and Intrinsic Proteins.- 1.6. The Scope of This Chapter.- 2. Methods of Dissociation of the Inner Membrane.- 2.1. Mechanical Disintegration.- 2.2. Chaotropic Agents.- 2.3. Detergents.- 2.4. Organic Solvents.- 2.5. Alkali, Salt, Chelating Agent, and Other Agents.- 3. Methods of Fractionation of Membrane Proteins.- 3.1. Centrifugation.- 3.2. Gel Filtration.- 3.3. Salting Out in the Presence of Detergents.- 3.4. Ion-Exchange Chromatography.- 3.5. Dialysis.- 3.6. Gel Adsorption.- 4. Analysis of the Membrane Components.- 4.1. Light Absorption.- 4.2. Gel Electrophoresis.- 4.3. Immunological Localization of Membrane Proteins.- 4.4. Radioimmunoassay of the Membrane Proteins.- 4.5. Enzyme Assay of the Membrane System.- 5. Purification of Extrinsic Proteins.- 5.1. Proteins of Energy Transfer.- 5.2. Proteins of Electron Transport.- 6. Purification of Intrinsic Proteins.- 6.1. Deficient Particles.- 6.2. Red-Green Separation.- 6.3. Proteins of Energy Transfer.- 7. Reassembly.- 7.1. Adsorption of Extrinsic Proteins on the Membrane.- 7.2. Formation of Vesicles with Intrinsic Proteins and Lipids.- 7.3. Ion Transport and Anisotropy of the Membrane.- 8. References.

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