ISBN-10:
1493991418
ISBN-13:
9781493991419
Pub. Date:
02/21/2019
Publisher:
Springer New York
Microbial Metabolic Engineering: Methods and Protocols

Microbial Metabolic Engineering: Methods and Protocols

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Overview

This volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part Two looks at the development of genetic tools for manipulating enzymes, biosynthetic pathways, and whole genomes; and Part Three covers the characterization of engineered microbes using targeted and global systems biology tools, as well as in silico models. Chapters explore topics such as leveraging enzyme promiscuity to construct novel biosynthetic pathways; assembling combinatorial multigene pathways for rapid strain optimization; applying 'omics technologies for identifying bottlenecks; and engineering nontraditional host organisms like cyanobacterium and Yarrowia lipolytica. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, Microbial Metabolic Engineering: Methods and Prools is a valuable resource for researchers and scientists interested in engineering and optimizing microbes for a variety of biotechnological applications.

Product Details

ISBN-13: 9781493991419
Publisher: Springer New York
Publication date: 02/21/2019
Series: Methods in Molecular Biology , #1927
Edition description: 1st ed. 2019
Pages: 252
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

1. Pathway and Gene Discovery from Natural Hosts and Organisms



Jing Ke and Yasuo Yoshikuni


2. Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity


James Jeffryes, Jonathan Strutz, Chris Henry, and Keith E.J. Tyo



3. Antisense RNA Elements for Downregulating Expression


Yaping Yang, Jian Wang, Ruihua Zhang, and Yajun Yan



4. Chemically Inducible Chromosomal Evolution (CIChE) for Multi-copy Metabolic Pathway Engineering

Aaron M. Love, Bradley W. Biggs, Keith E.J. Tyo, and Parayil Kumaran Ajikumar



5. Bacterial Expression of Membrane Associated Cyhrome P450s and their Activity Assay in Nanodiscs


Demetrios Maroutsos, Hannah Huff, and Aditi Das



6. Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae


Shuobo Shi, Youyun Liang, Ee Lui Ang, and Huimin Zhao



7. Assembly of Complex Pathways using Type IIs Restriction Enzymes


Sylvestre Marillonnet and Stefan Werner



8. Combinatorial Assembly of Multigene Pathways by Combining Single Strand Assembly with Golden Gate Assembly D. Bauwens, P. Coussement, J. Maertens, and M. De Mey



9. Checks and Balances with Use of the Keio Collection for Phenotype Testing


Sandra J. Aedo, Helena R. Ma, and Mark P. Brynildsen



10. Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp. PCC 6803


Jacob Sebesta, Allison Zimont, and Christie Ann Marie Peebles



11. Genetic Tools for Streamlined and Accelerated Pathway Engineering in Yarrowia lipolytica



Lynn Wong, Benjamin Holdridge, Jake Engel, and Peng Xu



12. Gas Chromatography-mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization



McKenzie L. Smith, April M. Miguez, and Mark P. Styczynski




13. Targeted Mass Spectrometry-based Proteomics Tools for Strain Optimization

Hsine-Chung Tseng and Christine Nicole S Santos



14. A Prool for the Construction and Curation of Genome-scale Integrated Metabolic and Regulatory Network Models


Sriram Chandrasekaran



15. 13C-Fingerprinting and Metabolic Flux Analysis of Microbial Metabolisms


Whitney Hollinshead, Lian He, and Yinjie J. Tang




16. A Genetic Screen for the Isolation of Mutants with Increased Flux in the Isoprenoid Pathway of Yeast


Manisha Wadhwa andAnand K Bachhawat

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