ISBN-10:
0132553732
ISBN-13:
9780132553735
Pub. Date:
09/25/1996
Publisher:
Prentice Hall Professional Technical Reference
Microbiology Lab Manual: Principles and Applications / Edition 1

Microbiology Lab Manual: Principles and Applications / Edition 1

by Stephen A. Norrell, Karen Messely

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Overview

Microbiology Lab Manual: Principles and Applications / Edition 1

This laboratory manual serves as a general introduction to the microbiology laboratory, including basic procedures and equipment. Its 36 exercises are divided into nine sections—Microscopic Technique, Microbial Diversity, Microbial Cultivation Techniques, Identification Techniques, Microbial Growth, Microbial Control, Clinical Microbiology, Virology, and Applied Microbiology. Questions are provided with each exercise to reinforce and challenge students' understanding of basic concepts and to require students to analyze or apply the material under discussion.

Product Details

ISBN-13: 9780132553735
Publisher: Prentice Hall Professional Technical Reference
Publication date: 09/25/1996
Edition description: Older Edition
Pages: 331
Product dimensions: 9.08(w) x 11.09(h) x 1.26(d)

Table of Contents

Preface vii
Laboratory Safety Procedure ix
Section I---Microscopic Technique
Introduction to Microscopy
1(10)
Preparation of Samears, Simple Stains, and Wet Mounts
11(8)
Differential Staining Procedures: Gram Stain, Acid-Fast Stain, Metachromatic-Granle Stain
19(12)
Additional Differential Staining Procedure: Demonstration of Becterial Cytology
31(10)
Calibration of the Microscope
41(8)
Cell Dimensions: Measurement of Cell Size
49(6)
Section II---Microbial Diversity
Survey of Microorganisms
55(10)
Section III---Microbial Cultivation Techniques
Isolation Techniques
65(14)
Oxygen Requirements of Microorganisms
79(8)
Physical Growth Requirements
87(8)
Selective and Differential Media
95(6)
Physiological Characteristics of Bacteria: Carbohydrate Metabolism
101(6)
Physiologic Characteristics of Bacteria: Reactions of Nitrogen Metabolism
107(6)
Physiologic Characteristics of Bacteria: Miscellaneous Reactions
113(6)
Section IV---Identification Techniques
Development and Use of Diagnostic Keys
119(8)
Use of Diagnostic Keys: Bacterial Unknowns
127(8)
Rapid Diagnostic Tests: Profile Analysis
135(12)
Section V---Microbial Growth
Microbial Population Counts: Microscopic Counting Methods
147(10)
Microbial Population Counts: Viable Cell Counts
157(10)
Spectrophotometric Methods
167(10)
Section VI---Microbial Control
Physical Control Methods
177(8)
UV Light as an Antimicrobial Agent
185(6)
Assay of Antimicrobial Agents: Disc-Diffusion Methods
191(8)
Assay of Antimicrobial Agents: Use-Dilution Methods
199(6)
Antibiotic Sensitivity Testing: The Kirby-Bauer Procedure
205(8)
Detection of Mutant Strains of Bacteria
213(8)
Section VII---Clinical Microbiology
Epidemiology
221(6)
Serological Reactions
227(12)
The Collection and Transport of Clinical Specimen
239(6)
Throat Cultures
245(10)
Urinary Tract Cultures
255(12)
Gastrointestinal Tract Cultures
267(10)
Lactobacillus Activity in Saliva
277(6)
Section VIII---Virology
Viral Population Counts: Plaque Counting and Plaque Morphology
283(6)
Section IX---Applied Microbiology
Food Microbiology
289(6)
Water Analysis: Standard Methods and Membrane Filter Techniques
295
Appendices
Appendix A Cultures Uses
A-1
Appendix B Media, Stains, Reagents, and Supplies
B-1
Appendix C References
C-1

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