ISBN-10:
1617376132
ISBN-13:
9781617376139
Pub. Date:
12/09/2010
Publisher:
Springer-Verlag New York, LLC
Microfluidic Techniques: Reviews and Protocols / Edition 1

Microfluidic Techniques: Reviews and Protocols / Edition 1

by Shelley D. Minteer

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Overview

Microfluidic Techniques: Reviews and Protocols / Edition 1

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The prools offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Product Details

ISBN-13: 9781617376139
Publisher: Springer-Verlag New York, LLC
Publication date: 12/09/2010
Series: Methods in Molecular Biology , #321
Edition description: Softcover reprint of hardcover 1st ed. 2006
Pages: 248
Product dimensions: 5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

Overview of Advances in Microfluidics and Microfabrication
Shelley D. Minteer and Christine M. Moore

Part I. Microfabrication Methods

Introduction to Microfabrication Techniques
Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou

Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds
Rabih Zaouk, Benjamin Y. Park, and Marc J. Madou

Fabrication of Microelectrodes Using the Lift-Off Technique
Benjamin Y. Park, Rabih Zaouk, and Marc J. Madou

Laser Ablation as a Fabrication Technique for Microfluidic Devices
Emanuel A. Waddell

Reversible, Room Temperature Bonding of Glass Devices for Microfluidics
Loranelle L. Lockyear

Part II. Biological Applications

Solid-Phase Extraction in Packed Beds on Glass Microdevices
Loranelle L. Lockyear

Fabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic Systems
Jin-Woo Choi

Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: Hydrogel Immunoassay
Gloria Thomas, Emad M. El-Giar, Laurie E. Locascio, and Michael J. Tarlov

Polymerase Chain Reaction in Miniaturized Systems: Big Progress in Little Devices
Kalee D. Spitzack and Victor M. Ugaz

Polymerase Chain Reaction on Microchips
Maria C. Carles and Nikolaus J. Sucher

A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes
Xiaojun Zhao, David T. Pierce, and Yanfu Huan

Microfluidic Ethanol Biobatteries on a Microchip
Shelley D. Minteer and Christine M. Moore

Microfluidic Chambers for Cell Migration and Neuroscience Research
Anne M. Taylor, Seog Woo Rhee, and Noo Li Jeon

Microtextured Polydimethylsiloxane Substrates for Culturing Mesenchymal Stem Cells
Erik T. K. Peterson and Ian Papautsky

Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip
Xiujun (James) Li and Paul C. H. Li

Microscale Integrated Sperm Sorter
Yaokuang Chung, Xiaoyue Zhu, Wei Gu, Gary D. Smith, and Shuichi Takayama

Index

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