Microtubules, in vitro 2E

Microtubules, in vitro 2E

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Product Details

ISBN-13: 9780124077577
Publisher: Elsevier Science
Publication date: 09/18/2013
Series: Methods in Cell Biology Series
Pages: 440
Product dimensions: 7.50(w) x 9.40(h) x 0.90(d)

Table of Contents

  1. Isolation of Antarctic fish tubulin and MAPs
  2. Making, isolating and characterizing tubulin alpha antibodies
  3. Techniques for labeling tubulin and drugs
  4. Single-particle 3D reconstruction methods for decorated and undecorated microtubules
  5. A new microtubule gliding assay and analysis of microtubule persistence length
  6. Characterization of gamma tubulin
  7. Biochemical and Structural Studies of the doublecortin family of MAPs
  8. Detection and quantification of microtubule detachment from centrosomes and spindle poles
  9. Regulation of tubulin expression by micro RNAs: Implications for Drug Resistance
  10. A portable magnetic tweezers device to visualize three-dimensional deformation of reconstituted microtubule networks
  11. Mitochondrial/microtubule interactions – mmbp1
  12. Going Solo: Measuring the motions of microtubules with an in vitro assay for TIRF microscopy
  13. Analysis of Microtubules and Motors in Isolated Axoplasm from the Squid Giant Axon
  14. Analysis of drug binding to microtubules and steady state microtubule growth/treadmilling by use of a sedimentation assay in vitro
  15. Specific recombinant antibody technology for the study of microtubule dynamics and regulatory proteins
  16. Tubulin-specific Chaperones: Components of a Molecular Machine that Assembles the aB Heterodimer
  17. G-protein tubulin interactions
  18. Katanin, a microtubule severing enzyme: expression, purification, and biophysical characterization
  19. Measuring tubulin polymerization in vitro – comparison of methods and possible artifacts
  20. Imaging microtubules in living plant cells
  21. How to use which antibody known in the field, what to expect from this antibody and how semi-quantitative data can be reliably obtained with antibody staining
  22. Preparation of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences
  23. Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry
  24. TBC
  25. Characterization of the mechanism of action of the covalent-binding microtubule-stabilizing agent zampanolide
  26. Studying kinetochore-fibre ultrastructure using correlative light-electron microscopy
  27. Fluorescence-based Assays for Microtubule Structure
  28. Yeast Tubulin-structure-function analysis
  29. Microtubule Binding SIm to simulate binding of proteins to polymers under different binding models

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