This book presents a compendium of molecular biology applications for the study of aquatic community ecology. The collection presents the diversity of approaches that have been used, and provides future directions for the study of 'molecular ecology' of aquatic communities, from viruses to fish, and in aquatic systems ranging from freshwater streams and lakes to estuaries and oceans. This collection of papers will provide a useful text and resource for upper-level undergraduate and graduate students in ecology, as well as for the researcher and educator.
Table of ContentsPreface. Molecular ecology of aquatic communities: where have we been, why are we here, and where are we going? J.P. Zehr. Plasmid Ecology of Marine Sediment Microbial Communities; J.P. Zehr, P.A. Sobecky. Use of the Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis to Study Diversity in Natural Virus Communities; S.M. Short, C.A. Suttle. Flow Cytometry in Molecular Aquatic Ecology; J.L. Collier, L. Campbell. Distribution of Microbial Assemblages in the Central Arctic Ocean Basin Studied by PCR/DGGE: Analysis of a Large Data Set; V.C. Ferrari, J.T. Hollibaugh. Bacterial Populations in Replicate Marine Enrichment Cultures: Assessing Variability in Abundance Using 16S rRNA-Based Probes; J.M. Gonzalez, et al. Diversity of Bacterial Communities in Adirondack Lakes: Do Species Assemblages Reflect Lake water Chemistry? B.A. Methè, J.P. Zehr. New Insights on Old Bacteria: Diversity and Function of Morphologically Conspicuous Sulfur Bacteria in Aquatic Systems; N.D. Gray, I.M. Head. The Distribution and Relative Abundance of Ammonia-Oxidizing Bacteria in Lakes of the McMurdo Dry Valley, Antarctica; M.A. Voytek, et al. Microscopic Detection of the Toluene Dioxygenase Gene and Its Expression Inside Bacterial Cells in Seawater Using Prokaryotic In Situ PCR; F. Chen, et al. Variability in bacterial community structure during upwelling in the Coastal Ocean; L.J. Kerkhof, et al. Application of Molecular Techniques to Addressing the Role of P as Key Effector in Marine Ecosystem; D.J. Scanlan, W.H. Wilson. Immunological and Molecular Probes to Detect Phytoplankton Responses to Environmental Stress in Nature; J. LaRoche, et al. Spatial Scale and the Diversity of Benthic Cyanobacteria and Diatoms in a Salina; U. Nubel, et al. A Rapid Method to Score Plastid Haplotypesin Red Seaweeds and Its Use in Determining Parental Inheritance of Plastids in the Red Alga Bostrychia (Ceramiales); G.C. Zuccarello, et al. Protistan Community Structure: Molecular Approaches for Answering Ecological Questions; D.A. Caron, et al. Molecular and Demographic Measures of Arsenic Stress in Daphnia pulex; C.Y. Chen, et al. Taxonomic and Systematic Assessment of Planktonic Copepods Using Mitochondrial COI Sequence Variation and Competitive, Species-Specific PCR; A. Bucklin, et al. Effects of Stress on Freshwater Invertrebrate Populations of Chironomus tentans: Assaying Sub-lethal Stress Using Heat Shock Protein 70 (HSP-70) Expression; N.K. Karouna-Renier, J.P. Zehr. RNA:DNA ratio and other nucleic acid-based indicators for Growth and Condition of Marine Fishes; L. Buckley, et al.