Molecular Genetics of Bacteria / Edition 3

Molecular Genetics of Bacteria / Edition 3

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Molecular Genetics of Bacteria / Edition 3

The Fourth Edition of this highly successful book provides an essential introduction to the molecular genetics of bacteria. Thoroughly revised and updated, Molecular Genetics of Bacteria now includes a much greater coverage of genomics, microarrays and proteomics. The text includes an enhanced treatment of the ways in which both classical and modern genetics have contributed to our understanding of how bacteria work. The focus of the book remains firmly on bacteria and will be invaluable to those students studying microbiology, biotechnology, molecular biology, biochemistry, genetics and related biomedical sciences.

Product Details

ISBN-13: 9780471977827
Publisher: Wiley
Publication date: 04/21/1998
Edition description: 3RD
Pages: 324
Product dimensions: 6.42(w) x 9.51(h) x 0.95(d)

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Table of Contents

1Nucleic Acid Structure and Function1
1.1Structure of nucleic acids1
1.1.3Hydrophobic interactions3
1.1.4Different forms of the double helix5
1.1.6Denaturation and hybridization10
1.1.7Orientation of nucleic acid strands11
1.2Replication of DNA12
1.2.1Unwinding and rewinding13
1.2.2Fidelity of replication: proof-reading13
1.3Chromosome replication and cell division14
1.4DNA repair19
1.4.1Mismatch repair19
1.4.2Excision repair19
1.4.3Recombination (post-replication) repair19
1.4.4SOS repair20
1.5Gene expression21
1.5.3Post-translational events32
1.6Gene organization34
2Mutation and Variation37
2.1Variation and evolution37
2.1.1Fluctuation test38
2.1.2Directed mutation in bacteria?40
2.2Types of mutations41
2.2.1Point mutations41
2.2.2Conditional mutants42
2.2.3Variation due to larger scale DNA alterations44
2.2.4Extrachromosomal agents and horizontal gene transfer44
2.4Restoration of phenotype47
2.4.1Reversion and suppression47
2.6Mechanisms of mutation50
2.6.1Spontaneous mutation50
2.6.2Chemical mutagens52
2.6.3Ultraviolet irradiation54
2.7Isolation and identification of mutants58
2.7.1Mutation and selection58
2.7.2Replica plating59
2.7.3Penicillin enrichment61
2.7.4Isolation of other mutants62
2.7.5Molecular methods62
3Regulation of Gene Expression67
3.1Gene copy number69
3.2Transcriptional control70
3.2.2Terminators, attenuators and anti-terminators77
3.2.3Induction and repression: regulatory proteins79
3.2.4Attenuation: trp operon87
3.2.5Two-component regulatory systems92
3.2.6Global regulatory systems94
3.2.7Feast or famine and the RpoS regulon95
3.2.8Quorum sensing95
3.3Translational control99
3.3.1Ribosome binding99
3.3.2Codon usage101
3.3.3Stringent response101
3.3.4Regulatory RNA102
3.3.5Phase variation102
4Genetics of Bacteriophages103
4.1Single-stranded DNA bacteriophages106
4.2RNA-containing phages: MS2109
4.3Double-stranded DNA phages110
4.3.1Bacteriophage T4110
4.3.2Bacteriophage lambda113
4.3.3Lytic and lysogenic regulation of bacteriophage lambda118
4.4Restriction and modification125
4.5Complementation and recombination128
4.6Why are bacteriophages important?130
4.6.1Phage typing131
4.6.2Phage therapy131
4.6.3Phage display132
4.6.4Bacterial virulence and phage conversion133
5.1Some bacterial characteristics are determined by plasmids137
5.1.1Antibiotic resistance137
5.1.2Colicins and bacteriocins138
5.1.3Virulence determinants138
5.1.4Plasmids in plant-associated bacteria139
5.1.5Metabolic activities139
5.2Molecular properties of plasmids141
5.2.1Plasmid replication and control143
5.3Plasmid stability154
5.3.1Plasmid integrity155
5.3.3Differential growth rate160
5.4Methods for studying plasmids161
5.4.1Associating a plasmid with a phenotype161
5.4.2Classification of plasmids163
6Gene Transfer165
6.2.1Mechanism of conjugation168
6.2.2The F plasmid173
6.2.3Conjugation in other bacteria174
6.3.1Specialized transduction180
6.4.1General (homologous) recombination181
6.4.2Site-specific and non-homologous (illegitimate) recombination186
6.5Mosaic genes and chromosome plasticity187
7Genomic Plasticity: Movable Genes and Phase Variation189
7.1Insertion sequences189
7.1.1Structure of insertion sequences189
7.1.2Occurrence of insertion sequences190
7.2.1Structure of transposons194
7.3Mechanisms of transposition197
7.3.1Replicative transposition197
7.3.2Non-replicative (conservative) transposition200
7.3.3Regulation of transposition201
7.3.4Activation of genes by transposable elements203
7.3.5Mu: a transposable bacteriophage204
7.3.6Conjugative transposons and other transposable elements205
7.4Phase variation205
7.4.1Variation mediated by simple DNA inversion207
7.4.2Variation mediated by nested DNA inversion208
7.4.3Antigenic variation in the gonococcus208
7.4.4Phase variation by slipped strand mispairing211
7.4.5Phase variation mediated by differential DNA methylation214
8Genetic Modification: Exploiting the Potential of Bacteria215
8.1Strain development215
8.1.1Generation of variation215
8.1.2Selection of desired variants216
8.2Overproduction of primary metabolites216
8.2.1Simple pathways217
8.2.2Branched pathways218
8.3Overproduction of secondary metabolites220
8.4Gene cloning221
8.4.1Cutting and joining DNA222
8.4.2Plasmid vectors223
8.4.4Bacteriophage lambda vectors225
8.4.5Cloning larger fragments227
8.4.6Bacteriophage M13 vectors229
8.5Gene libraries229
8.5.1Construction of genomic libraries229
8.5.2Screening a gene library231
8.5.3Construction of a cDNA library233
8.6Products from cloned genes234
8.6.1Expression vectors234
8.6.2Making new genes236
8.6.3Other bacterial hosts239
8.6.4Novel vaccines241
8.7Other uses of gene technology242
9Genetic Methods for Investigating Bacteria245
9.1Metabolic pathways245
9.2Microbial physiology247
9.2.1Reporter genes249
9.2.3Cell division251
9.2.4Motility and chemotaxis252
9.2.5Cell differentiation253
9.3Bacterial virulence257
9.3.1Wide range mechanisms of bacterial pathogenesis257
9.3.2Detection of virulence genes259
9.4Specific mutagenesis262
9.4.1Gene replacement262
9.4.2Antisense RNA264
9.5Taxonomy, evolution and epidemiology264
9.5.1Molecular taxonomy264
9.5.2Diagnostic use of PCR267
9.5.3Molecular epidemiology267
10Gene Mapping to Genomics273
10.1Gene mapping273
10.1.1Conjugational analysis273
10.1.2Co-transformation and co-transduction276
10.1.3Molecular techniques for gene mapping277
10.2Gene sequencing279
10.2.1DNA sequence determination281
10.2.2Genome sequencing282
10.2.3Comparative genomics285
10.3Physical and genetic maps289
10.3.1Deletions and insertions290
10.3.2Transposon mutagenesis290
10.3.3Gene replacement292
10.3.4Site-directed mutagenesis292
10.4Analysis of gene expression292
10.4.1Transcriptional analysis293
10.4.2Translational analysis296
10.4.3Systematic analysis of gene function300
Appendix AFurther Reading301
Appendix BAbbreviations305
Appendix CGlossary309
Appendix DEnzymes323
Appendix EGenes327
Appendix FStandard Genetic Code331
Appendix GBacterial Species333

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