Molecular Protocols in Transfusion Medicine

Molecular Protocols in Transfusion Medicine

NOOK Book(eBook)

$92.99 $109.00 Save 15% Current price is $92.99, Original price is $109. You Save 15%.
View All Available Formats & Editions

Available on Compatible NOOK Devices and the free NOOK Apps.
WANT A NOOK?  Explore Now

Product Details

ISBN-13: 9780080536903
Publisher: Elsevier Science
Publication date: 04/27/2000
Sold by: Barnes & Noble
Format: NOOK Book
Pages: 186
File size: 5 MB

Table of Contents

Preface

List of Abbreviations

Part 1: Introduction

1.1 Background

1.2 Aim, Scope and Purpose of the Book

1.3 Organization of the Book

1.4 Terminology

1.5 Regulatory Compliance

1.6 References

Part 2: General Considerations

2.1 Clinical Information

2.2 Basic Principles of Molecular Analysis

2.3 Troubleshooting

2.4 References

Part 3: Protocols

3.1 Sample Shipment and Storage

3.2 Reagents

3.3 Harvesting WBCs by RBC Sedimentation

3.4 Short-term Cell Cultures from Amniotic Fluid

3.5 Cultured Amniocyte Cell Harvesting

3.6 Cell Preparation and DNA Extraction

3.7 Assessment of DNA Quality, Quantity and Purity

3.8 PCR Amplification

3.9 Restriction Enzyme Digestion for PCR-RFLP Analysis

3.10 Allele-specific PCR

3.11 Agarose Gel Preparation and Electrophoresis

3.12 Acrylamide Gel Preparation and Electrophoresis

3.13 Single-strand Conformation Polymorphism (SSCP)

3.14 Optimizing a PCR

3.15 Gene Zygosity Assessment by Quantitative PCR Amplification

3.16 VNTR and STR Analysis by PCR Amplification

3.17 Multiplex PCR Analysis

3.18 Southern Analysis

3.19 References

Part 4: Red Cell Blood Groups

4.1 Terminology

4.2 Clinical Applications

4.3 Situations where Genotype and Phenotype do not Agree

4.4 References

4.5 Facts Sheets, Gene Maps and Molecular Protocols

Part 5: Platelet Blood Groups

5.1 Terminology

5.2 Clinical Applications

5.3 References

5.4 Facts Sheets, Gene Maps and Molecular Protocols

Part 6: Neutrophil Blood Groups

6.1 Terminology

6.2 Clinical Applications

6.3 References

6.4 Facts Sheets, Gene Maps and Molecular Protocols

Part 7: Appendices

7.1 Database Information

7.2 Glossary

7.3 References

7.4 Works Sheets and Request Forms

Index

Useful Tables

Customer Reviews

Most Helpful Customer Reviews

See All Customer Reviews