Neisseria meningitidis: Advanced Methods and Protocols

Neisseria meningitidis: Advanced Methods and Protocols

by Myron Christodoulides (Editor)

Hardcover(2012)

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Product Details

ISBN-13: 9781617793455
Publisher: Springer-Verlag New York, LLC
Publication date: 10/13/2011
Series: Methods in Molecular Biology , #799
Edition description: 2012
Pages: 427
Product dimensions: 7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

1. Neisseria meningitidis: Biology, Microbiology, and Epidemiology

Nadine G. Rouphael and David S. Stephens

2. Classification and Pathogenesis of Meningococcal Infections

Petter Brandtzaeg and Marcel van Deuren

3. Detection of Neisseria meningitidis in Cerebrospinal Fluid Using a Multiplex PCR and the Luminex Detection Technology

Jens Kjølseth Møller

4. Generating Knock-Out and Complementation Strains of Neisseria meningitides

Vincent van Dam and Martine P. Bos

5. Identification and Functional Characterization of sRNAs in Neisseria meningitides

Yvonne Pannekoek and Arie van der Ende

6. Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins

Muhammad Saleem, Jeremy Moore, and Jeremy P. Derrick

7. Genome-Scale Metabolic Models: Reconstruction and Analysis

Gino J.E. Baart and Dirk E. Martens

8. TMT Labeling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome

Karsten Kuhn, Christian Baumann, Jan Tommassen, and Thorsten Prinz

9. Meningococcal Ligands and Molecular Targets of the Host

Darryl J. Hill and Mumtaz Virji

10. In Vivo Imaging of Meningococcal Disease Dynamics

Hong Sjölinder and Ann-Beth Jonsson

11. Methods for Studying Neisseria meningitidis Biofilms

Michael A. Apicella, Jianqiang Shao, and R. Brock Neil

12. A Laminar-Flow Chamber Assay for Measuring Bacterial Adhesion Under Shear Stress

Magali Soyer and Guillaume Duménil

13. Techniques to Measure Pilus Retraction Forces

Nicolas Biais, Dustin Higashi, Magdalene So, and Benoit Ladoux

14. Human Dendritic Cell Culture and Bacterial Infection

Hannah E. Jones, Nigel Klein, and Garth L.J. Dixon

15. Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry (H/DX-MS) to Investigate Ligand-Receptor Interactions

Jessmi M.L. Ling, Leslie Silva, David C. Schriemer, and Anthony B. Schryvers

16. Visualizing PAMP-PRR Interactions Using Nanoscale Imaging

Kathy Triantafilou and Martha Triantafilou

17. Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells

Biju Joseph, Matthias Frosch, Christoph Schoen, and Alexandra Schubert-Unkmeir

18. The Use of the Pan-Neisseria Microarray and Experimental Design for Transcriptomics Studies of Neisseria

Nigel J. Saunders and John K. Davies

19. Analysis of Parameters Associated with Prevention of Cellular Apoptosis by Pathogenic Neisseriae and Purified Porins

Paola Massari and Lee M. Wetzler

20. Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach

Jeannette N. Williams, Myron Christodoulides, and John E. Heckels

21. Antigen Identification Starting from the Genome: A "Reverse Vaccinology" Approach Applied to MenB

Emmanuelle Palumbo, Luigi Fiaschi, Brunella Brunelli, Sara Marchi, Silvana Savino, and Mariagrazia Pizza

22. A DNA Vaccine Strategy for Effective Antibody Induction to Pathogen-Derived Antigens

Jason Rice and Myron Christodoulides

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