Neurocytochemical Methods

Neurocytochemical Methods

Paperback(Softcover reprint of the original 1st ed. 1991)

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Product Details

ISBN-13: 9783642843006
Publisher: Springer Berlin Heidelberg
Publication date: 01/19/2012
Series: Nato ASI Subseries H: , #58
Edition description: Softcover reprint of the original 1st ed. 1991
Pages: 335
Product dimensions: 6.69(w) x 9.53(h) x 0.03(d)

Table of Contents

Development and application of antibodies to primary (DA, L-DOPA) and secondary (cGMP) messengers: A technical report.- Hodology.- Transmitter-related retrograde labeling and immunohistochemistry to localize amino acid transmitters in neural pathways and cells.- Ultrastructural identification and quantification of neuroactive amino acids.- Video-microscopic techniques to study axonal transport, neuronal fine structure and cytochemical parameters in live neurons.- Investigating the three-dimensional morphology of fluorescence-labelled neurons using confocal laser scanning microscopy.- Chairman’s report.- Detection of Specific Molecules.- Combined cytolabeling methods for investigating chemo-specific neuronal interactions and transmitter co-localization.- Molecular neuroanatomy of synapses, cells and systems in the brain.- Regulated expression of transcription factor and neurotransmitter genes in neural populations: studies using in situ hybridization with radiolabeled oligonucleotide probes.- The use of markers to study the regulation of glial cells in the PNS and CNS.- Chairlady’s report.- Quantitative Aspects.- Applications to the study of acetylcholine release of the choline oxidase acetylcholine assay.- Morphometric evaluation of populations of neuronal profiles in the central nervous system.- Positron emission tomography: functional neuroanatomy in vivo.- Microfluorometric imaging of time and space dependent calcium concentration changes in mammalian neurons.- Chairman’s report.- Receptors.- Immunocytochemistry of neuropeptide receptors using anti-idiotypic antibodies and antibodies to peptides encoded by complementary RNA.- New approaches for receptor analysis: histochemistry of the GABA/BZ receptors in the rat CNS.- Chairman’s report.- Short Papers.- Extracellular dyes induce photodegeneration and photopermeabilization of vertebrate and invertebrate neurons.- A method to study projections of GABAergic neurons using deletion of the GABA immunostaining by metallic ions.- Ultrastructural immunocytochemical localization of choline acetyltransferase in sympathetic ganglia.- A dual immunoperoxidase labelling method to study the ultrastructural relationships between choline acetyltransferase- and neuropeptide Y-containing neurons in the rat striatum.- Characterization of microglia and brain macrophages.- Visualization and quantification of 5-HT1A receptors in the rat brain by radioimmunohistochemistry.- Anti-idiotypic antibodies against opioid receptors: first ultrastructural evidence for a presynaptic inhibition by opioids in the dorsal horn of the rat spinal cord.- Antibodies to complementary peptide for labelling substance P receptors in the dorsal horn of the rat spinal cord for both light and electron microscopy.- Comparative ultrastructural localization of neuropeptide mRNAs using tritiated and biotinylated oligodeoxynucleotide probes.- Use of in situ hybridization and immunohistochemistry to study the effects of intranigral injections of colchicine on enkephalin- and substance P-containing neurons of the limbic forebrain.- Chairman’s report.- Author Index.

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