This volume explores the latest techniques used by researchers to help them better understand the NMDAR structure-function relations, principles, and rules that govern how NMDARs operate in brain processing. The volume includes a detailed introductory chapter describing the field and is divided into three parts. Topics covered in the volume include: quantification of NMDAR subunit genes expression by qRT-PCR; detection of NMDARs antibodies in encephalitis; recombinant channels in host cells using a fast agonist application system; GluNs detection and functions in microglial cells; and NMDARs as voltage sensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, NMDA Receptors: Methods and Protocols is a valuable resource that discusses the diversity of the currently-used methods, the importance of NMDARs and their complexity, and the progress that has already been made. This book will be of interest to scientists, clinicians, and industry professionals working in this field.
Table of Contents
Preface… Table of Contents… Contributing Authors… 1. NMDA Receptors in the Central Nervous SystemKasper B. Hansen, Feng Yi, Riley Perszyk, Frank S. Menniti, and Stephen F. TraynelisPart I NMDAR Subunits Expression and Mutations2. Quantification of NMDAR Subunit Genes Expression by qRT-PCREmilie Pallesi-Pocachard3. Genetic and Functional Analysis of GRIN2A in Tumor SamplesTodd D. Prickett, Jared J. Gartner, and Yardena Samuels4. Detection of NMDARs Antibodies in EncephalitisMatteo Gastaldi, Patrick Waters, and Angela VincentPart II Studying Structure-Function Relations in NMDARs5. MagnetofectionTM of NMDA Receptor Subunits GluN1 and GluN2A Expression Vectors in Non-Neuronal Host CellsNadine Bruneau and Pierre Szepetowski6. Transfection in Primary Cultured Neuronal CellsKatie F. M. Marwick and Giles E. Hardingham7. Selective Cell-Surface Expression of Triheteromeric NMDA ReceptorsFeng Yi, Stephen F. Traynelis, and Kasper B. Hansen8. Functional Analysis of Recombinant Channels in Host Cells using a Fast Agonist Application SystemAndrei Rozov and David Jappy9. GluN2B Subunit Labeling with Fluorescent Probes and High-Resolution Live ImagingC. Perrio, O. Nicole, and A. Buisson10. Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-LinkersMeilin Tian and Shixin YePart III Analysis of NMDAR Function/Activity11. Gene Targeted Mice with Conditional Knock-In (-Out) of NMDAR MutationsRolf Sprengel, Ahmed Eltokhi, and Frank N. Single12. Electrophysiological Investigation of NMDA Current Properties in Brain SlicesAlexandre Pons Bennaceur and Natalia Lozovaya13. Analysis of Functional NMDA Receptors in AstrocytesFrank Kirchhoff14. GluNs Detection and Functions in Microglial CellsStephane Peineau, Vincent Degos, Catherine Verney, and Pierre Gressens15. NMDA Receptor Activity in Circulating Red Blood Cells: Methods of DetectionAsya Makhro, Lars Kaestner, and Anna Bogdanova16. NMDA Receptors as Voltage SensorsRoustem Khazipov17. Development of a Computational Approach/Model to Explore NMDA Receptors FunctionsA. Florence Keller, Jean-Marie C. Bouteiller, and Theodore W. Berger