Nuclear Reprogramming: Methods and Protocols

Nuclear Reprogramming: Methods and Protocols

by Steve Pells (Editor)

Paperback(Softcover reprint of hardcover 1st ed. 2006)

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Product Details

ISBN-13: 9781617375309
Publisher: Springer-Verlag New York, LLC
Publication date: 12/20/2010
Series: Methods in Molecular Biology , #325
Edition description: Softcover reprint of hardcover 1st ed. 2006
Pages: 334
Product dimensions: 5.98(w) x 9.02(h) x (d)

Table of Contents

Nuclear Transplantation in Xenopus
John B. Gurdon

Nuclear Transfer in Sheep
William A. Ritchie

Protocols for Nuclear Transfer in Mice
Shaorong Gao

Isolation of Stromal Stem Cells From Human Adipose Tissue
Andrew C. Boquest, Aboulghassem Shahdadfar, Jan E. Brinchmann, and Philippe Collas

Nuclear Reprogramming by Cell Fusion
Robert H. Broyles, Austin C. Roth, Mairead Todd, and Visar Belegu

Polyethylene Glycol-Mediated Cell Fusion
Jian Yang and Ming Hong Shen

Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells
Masako Tada and Takashi Tada

Quantification of Cell Fusion by Flow Cytometry
Stephen Sullivan, Martin Waterfall, Ed J. Gallagher, Jim McWhir, and Steve Pells

Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts
Anne-Mari Håkelien, Kristine G. Gaustad, and Philippe Collas

Transgenic Systems in Nuclear Reprogramming
Megan Munsie, Peter Mountford, and Jennifer Nichols

Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos
Fátima Santos and Wendy Dean

Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies
Rong Wu, Anna V. Terry, and David M. Gilbert

Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones
Dean H. Betts, Steven Perrault, Lea Harrington, and W. Allan King

Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells
Cornelia Wiese, Gabriela Kania, Alexandra Rolletschek, Przemyslaw Blyszczuk, and Anna M. Wobus

Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens
Bruce A. Fenderson, Maria P. De Miguel, April D. Pyle, and Peter J. Donovan

Analysis of the Nucleolar Compartment of the Nucleus as anIndicator of Nuclear Reprogramming After Nuclear Transfer
Jacques-E. Fléchon

Methylation-Sensitive Polymerase Chain Reaction
Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young

Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis
Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang

Chromatin Immunoprecipitation Assay for Mammalian Tissues
Fiona B. Turner, Wang L. Cheung, and Peter Cheung

Histone Modifications and Transcription Factor Binding on Chromatin: ChIP-PCR Assays
Jaejoon Won and Tae Kook Kim

In Vivo Genomic Footprinting Using LM-PCR Methods
Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer

Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease
Pascal Lefevre and Constanze Bonifer

Index

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