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Odontogenesis: Methods and Protocols

Odontogenesis: Methods and Protocols

by Petros Papagerakis (Editor)

Hardcover(1st ed. 2019)

$279.99
Available for Pre-Order. This item will be available on March 10, 2019

Product Details

ISBN-13: 9781493990115
Publisher: Springer New York
Publication date: 03/10/2019
Series: Methods in Molecular Biology , #1922
Edition description: 1st ed. 2019
Pages: 586
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Part I: Protocols for Establishment of Dental Cells Lines and Animal Models


1. Micro-dissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors


Hidemitsu Harada and Keishi Otsu




2. Establishment of an Immortalized Mouse Bmp2 Knock--Out Dental Papilla Mesenchymal Cell Line

Wen'an Xu and Shuo Chen



3. Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells


Elizabeth Guirado, Youbin Zhang, and Anne George



4. Isolation of Dental Stem Cell Enriched Populations from Continuously Growing Mouse Incisors


Anamaria Balic



5. Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentogenesis


Yan Jing, Chaoyuan Li, and Jian Q. Feng



6. Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-mesenchymal Interactions


Lucia Jimenez-Rojo and Thimios Mitsiadis



7. Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, Primary Teeth Stem Cells: Isolation, Characterization and Expansion for Tissue Engineering


Mey Al-Habib and George T.-J. Huang




Part II: Protocols for Dental Stem Cells and Tissue Regeneration


8. Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal Cells Subpopulations with Multiparametric Flow Cytometry


Maxime Ducret, Jean-Christophe Farges, Marielle Pasdeloup, Emeline Perrier-Groult, Andreas Mueller, Frédéric Mallein-Gerin, and Hugo Fabre



9. Dental Pulp Stem Cells: Isolation, Characterization, Expansion and Odontoblast Differentiation for Tissue Engineering



Qing, Dong; Wang, Yuanyuan; Mohabatpour, Fatemeh; Zheng, Li; Papagerakis, Silvana; Chen, Daniel; and Papagerakis, Petros



10. In vitro Analysis of Intramolecular Signaling Events in PDLSCs using Confocal and TIRF Microscopy


Annette Merkel and Anne George



11. A Mouse Model to Study Reparative Dentinogenesis


Babb RC, Chandrasekaran D, Zaugg LK, and Sharpe PT



12. Multiwalled Carbon Nanotubes for Dental Applications


Petros Kechagioglou;Eleftherios Andriotis; Petros Papagerakis; and Silvana Papagerakis



13. Peptide-mediated Biomimetic Regrowth of Human Enamel in situ


Kaushik Mukherjee, Qichao Ruan, and Janet Moradian-Oldak




14. Bioengineering Tooth Bud Constructs using GelMA Hydrogel

Elizabeth E. Smith and Pamela C. Yelick



15. Whole-mount In Situ Hybridization of Mouse Embryos Using DIG-labeled RNA Probes


Jingyi Wu and Xiaofang Wang




Part III: Protocols for Studying Gene and Protein Expression



16. In Situ Hybridization on Mouse Paraffin Sections Using DIG-labeled RNA Probes


Jingyi Wu, Jian Q. Feng, and Xiaofang Wang



17. Methods for in situ Proteins Visualization in Dental Mineralized Tissues


D. Hotton, A. Berdal, and A. Bolanos



18. In Situ Hibridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection


Lignon G, Hotton D, Berdal A, and Bolanos A




19. Immunofluorescence Procedure for Developing Enamel Tissues


Xu Yang and Elia Beniash



20. Silver-albumin Tissue Staining Protocol to Visualize Odontogenesis in Whole Embryos


Julia C. Boughner and David M. L. Cooper



21. Isolation of SIBLING Proteins from Bone and Dentin Matrix


Jingyi Wu and Xiaofang Wang



22. Immunohistochemical Co-localization of Amelogenin and Ameloblastin in Developing Enamel Matrix


Rucha Arun Bapat and Janet Moradian-Oldak



23. The Expression and Purification of Recombinant Mouse Ameloblastin in e-coli


Jingtan Su, Rucha Bapat, and Janet Moradian-Oldak


Part IV: Protocols for Biochemistry and Imaging



24. Protocols for Studying Formation and Mineralization of Dental Tissues in vivo



Extraction Protocol for Isolating Dentin Matrix Proteins from Developing Teeth


Yasuo Yamakoshi, Jan C-C Hu, Mari M. Saito, and James P. Simmer



25. Purification of Developing Enamel Matrix Proteins using Preparative SDS PAGE


Steven J. Brookes and Claire M. Gabe



26. Using ImageJ (FIJI) to Analyse and Present X Ray CT Images of Enamel


Steven J. Brookes



27. Scanning Electron Microscopy (SEM) Methods for Dental Enamel


Steinar Risnes, Muhammad Saeed, and Amer Sehic



28. Microcomputed Tomography Imaging in Odontogenesis Studies


Kostas Verdelis and Phil Salmon



29. Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) for the Examination of Dental Hard Tissues


Victor E. Arana-Chavez and Leticia S. Castro-Filice



30. Rodent Dental Fluorosis Model--Extraction of Enamel Organ from Rat Incisors


M. Suzuki and J. D. Bartlett




Part V: Protocols to Study Dental Diseases



31. Three Dimensional Assessment of Crown Size and Eruption Space for Developing Third Molars: Data Collection Techniques Based on Cone-beam Computed Tomography (CBCT)


D.F. Marchiori, G.V. Packota, and J.C. Boughner



32. Protocols to Study Dental Caries In Vitro: Microbial Caries Models


Bennett T. Amaechi, Livia M. A. Tenuta, Antonio P. Ricomini Filho, and Jaime A. Cury



33. In vitro Caries Models for the Assessment of Novel Restorative Materials


Sulaiman Ghandourah, Basma; Lefkelidou, Anna;Said, Raed; Chatzistavrou, Xanthippi; Flannagan, Susan; Gonzáles-Cabezas, Carlos; Fenno, Christopher J.; Zheng, Li;Papagerakis, Silvana; and Papagerakis, Petros



34. Protocols to Study Dental Caries In Vitro: pH Cycling Models


Bennett T. Amaechi



35. In Vivo Rodent Models for Studying Dental Caries and Pulp Disease


Hsiao, June; Wang, Yuanyuan;Liu, Ruirui; Zheng, Li; Said; Raed; Hadjiyski, Lubomir; Cha, Heekon; Botero, Tatiana; Chatzistavrou, Xanthippi; Qing, Dong;Papagerakis, Silvana; Papagerakis, Petros




36. Protocol GenoDENT: Implementation of a New NGS Panel for Molecular Diagnosis of Genetic Disorders with Orodental Involvement


Tristan Rey, Julien Tarabeux, Bénédicte Gerard, Marion Delbarre, Antony Le Béchec Corinne Stoetzel, Megana Prasad, Virginie Laugel-Haushalter, Marzena Kawczynski, Jean Muller, Jamel Chelly, Hélène Dollfus, Marie-Cécile Manière, and Agnès Bloch-Zupan




Part VI: Protocols for Genetic, Epigenetic and Clinical Studies


37. Protocols for Genetic and Epigenetic Studies of Rare Diseases Affecting Dental Tissues


Bruna Rabelo Amorim, Pollyanna Almeida Costa dos Santos, Caroline Lourenço de Lima, Denise Carleto Andia, Juliana Forte Mazzeu, and Ana Carolina Acevedo



38. Protocols, Methods, and Tools for Genome-wide Association Studies (GWAS) of Dental Traits


Cary S. Agler, Dmitry Shungin, Andrea G. Ferreira Zandoná, Paige Schmadeke,Patricia V. Basta, Jason Luo, John Cantrell,Thomas D. Pahel Jr., Beau Meyer, John R. Shaffer, Arne S. Schäefer, Kari E. North, and Kimon Divaris



39. Measurement of Early Childhood Oral Health for Research Purposes: Dental Caries Experience and Developmental Defects of the Enamel in the Primary Dentition


Jeannie Ginnis, Andrea G. Ferreira Zandoná, Gary D. Slade, John Cantrell, Mikafui E. Antonio,Bhavna T. Pahel, Beau D. Meyer, and Kimon Divaris



40. The Supragingival Biofilm in Early Childhood Caries: Clinical and Laboratory Protocols and Bioinformatics Pipelines Supporting Oral Metagenomics, Metatranscriptomics and Metabolomics Studies of the Oral Microbiome


Kimon Divaris, Dmitry Shungin,Adaris Rodríguez-Cortés, Patricia V. Basta, Jeff Roach,Andrea G. Ferreira Zandoná, Jeannie Ginnis, Sivapriya Ramamoorthy,Jakub Kwintkiewicz, Natasha Butz, and M. Andrea Azcarate-Peril



41. Saliva and Gingival Crevicular Fluid (GCF) Collection for Biomarkers Screening



Petros Papagerakis,Raed Said, Doohak Kim, Amber A. Ehlert, Kevin K. M. Chung, and Silvana Papagerakis