Organ Regeneration: Methods and Protocols

Organ Regeneration: Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2013)

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Product Details

ISBN-13: 9781493960088
Publisher: Springer-Verlag New York, LLC
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #1001
Edition description: Softcover reprint of the original 1st ed. 2013
Pages: 377
Product dimensions: 7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

1. Isolation and Characterization of Human Adipose-Derived Stem Cells for Use in Tissue Engineering

Benjamin M. Buehrer and Bentley and Cheatham

2. Isolation of Smooth Muscle Cells from Bladder for Generation of Engineered Urologic Organs

Darell W. McCoy

3. Isolation of Urothelial Cells from Bladder Tissue

Namrata Sangha

4. Isolation of Pulsatile Cell Bodies from Esophageal Tissue

John W. Ludlow, Joydeep Basu, Christopher W. Genheimer, Kelly I. Guthrie, and Namrata Sangha

5. Cell Isolation Through Whole Liver Perfusion and Preparation of Hepatocytes for Cytochrome P450 Analysis

Sonya O. Meheux

6. Ex Vivo Culture and Separation of Functional Renal Cells

Andy Bruce, Kelly Guthrie, and Rusty Kelley

7. Isolation and Myogenic Differentiation of Mesenchymal Stem Cells (MSC) for Urologic Tissue Engineering

Rongpei Wu, Guihua Liu, Shantaram Bharadwaj, and Yuanyuan Zhang

8. Xeno-Free Adaptation and Culture of Human Pluripotent Stem Cells

Tori Sampsell-Barron

9. Reverse Engineering Life: Physical and Chemical Mimetics for Controlled Stem Cell Differentiation into Cardiomyocytes

Gary R. Skuse and Kathleen A. Lamkin-Kennard

10. Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine

Teresa Bora Burnette and Andrew T. Bruce

11. Preparation and Evaluation of Natural Scaffold Materials for Kidney Regenerative Applications

Monica A. Serban, Toyin A. Knight, and Richard G. Payne

12. Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration

Adelola O. Oseni, Peter E. Butler, and Alexander M. Seifalian

13. Electrospinning Tubular Scaffolds with Tissue-Like Mechanical Properties and Biomimetic Surface Features

Scott Rapoport

14. Design, Fabrication, and Preparation of Synthetic Scaffolds for Urologic Tissue Engineering

Richard G. Payne and Toyin A. Knight

15. Characterization of a PGA Based Scaffold for Use in a Tissue Engineered Neo-Urinary Conduit

Toyin A. Knight and Richard G. Payne

16. Migration Assay to Evaluate Cellular Interactions with Biomaterials for Tissue Engineering/Regenerative Medicine Applications

Kelly Guthrie, Namrata Sangha, Christopher Genheimer, Joydeep Basu, and John W. Ludlow

17. Care of Rodent Models used for Pre-Clinical Evaluation of Tissue Engineered/Regenerative Medicine Product Candidates

Kim L. Mihalko

18. Bioreactor Design Considerations for Hollow Organs

Jeff Fish, Craig Halberstadt, Darell McCoy, and Neil Robbins

19. Construction of a Multicoaxial Hollow Fiber Bioreactor

Randall McClelland, Katherine Tech, and Jeffrey M. Macdonald

20. Microencapsulation of Pancreatic Islets for Use in a Bioartificial Pancreas

Emmanuel C. Opara, John P. McQuilling, and Alan C. Farney

21. Bioengineered Skin Substitutes

Pedro Lei, Hui You, and Stelios T. Andreadis

22. Formulation of Selected Renal Cells for Implantation into a Kidney

Craig Halberstadt, Neil Robbins, Darell McCoy, Kelly Guthrie, Andrew Bruce, Toyin Knight, and Richard Payne

23. Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold

Pedro M. Baptista, Dipen Vyas, Emma Moran, Zhan Wang, and Shay Soker

24. A "Living Bioreactor" for the Production of Tissue Engineered Small Intestine

Daniel E. Levin, Frederic G. Sala, Erik R. Barthel, Allison L. Speer, Xiaogang Hou, Yasuhiro Torashima, and Tracy C. Grikscheit

25. Tissue Engineering of Esophagus and Small Intestine in Rodent Injury Models

Joydeep Basu, Kim Mihalko, Elias Rivera, Kelly I. Guthrie, Christopher Genheimer, Namrata Sangha, and John W. Ludlow

26. Scanning Electron Microscopy Evaluation of Endothelialized Tissue Engineered Constructs

Sandra L. Johnson

27. Genotypic and Phenotypic Analysis of in vivo Tissue Regeneration in an Animal Model

Christopher W. Genheimer

28. Histological Evaluation of Tissue Regeneration Using Biodegradable Scaffold Seeded by Autologous Cells for Tubular/Hollow Organs Applications

Elias A. Rivera and Manuel J. Jayo

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