Pub. Date:
Springer-Verlag New York, LLC
Osteoporosis: Methods and Protocols / Edition 1

Osteoporosis: Methods and Protocols / Edition 1

by Jennifer Westendorf


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Product Details

ISBN-13: 9781588298287
Publisher: Springer-Verlag New York, LLC
Publication date: 04/16/2008
Series: Methods in Molecular Biology , #455
Edition description: 2008
Pages: 354
Product dimensions: 6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Table of Contents
(* indicates corresponding author)

In vitro models

1. Isolation and Culture of Rodent Osteoprogenitor Cells
Thomas A. Owen* and Lydia C. Pan

2. Osteoclast Culture and Resorption Assays
Elizabeth W. Bradley and Merry Jo Oursler*

3. Assessing New Bone Formation in Neonatal Calvarial Organ Cultures
Khalid S. Mohammad, John M. Chirgwin and Theresa Guise*

4. Detection of Apoptosis of Bone Cells In Vitro
Teresita Bellido*and Lilian I. Plotkin

In vivo rodent models

5. In Vivo Parathyroid Hormone Treatments and mRNA Isolation and Analysis
Xin Li, Ling Qin and Nicola C. Partridge*

6. Assessment of Bone Formation Capacity Using In Vivo Transplantation Assays: Procedure and Tissue Analysis
Badem M. Abdalleh, Nicholas Ditzel and Moustapha Kassem*

7. Ossicle and Vossicle Implant Model Systems
Glenda J. Pettway and Laurie K. McCauley*

8. Utility of the Ovariectomized (OVX) Rat as a Model for Human Osteoporosis In Drug Discovery
Yogendra P. Kharode*, Michael C. Sharp and Peter V. N. Bodine

9. Orchidectomy (ORX) Models of Osteoporosis

Stéphane Blouin, Hélène Libouban, Marie Françoise Moreau and Daniel Chappard*

Methods of gene delivery

10. Gene Delivery by Adenoviruses
Renny Franceschi* and Chunxi Ge

11. Lentiviral Delivery of shRNA Constructs into Osteoblasts
Marc N. Wein, Dallas C. Jones and Laurie H. Glimcher*

12. Gene Delivery by Retroviruses
Valerie Deregowski and Ernesto Canalis*

Molecular and GeneticTechniques

13. Chromatin Immunoprecipitation (ChIP) Assays: Application of ChIP-on-chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells
Margaret van der Deen, Mohammad Q. Hassan, Jitesh Pratap, Nadiya M. Teplyuk, Daniel W. Young, Amjad Javed, Sayyed K. Zaidi, Jane B. Lian, Martin Montecino, Janet L. Stein, Gary S. Stein and Andre J. van Wijnen*

14. Identification of Transcription Factor Target Genes by ChIP Display (CD)
Artem Barski, Steve Pregizer and Baruch Frenkel*

15. Application of Laser Capture Microdissection (LCM) Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo
Dafna Benayahu*, Rina Socher, and Irena Shur

16. Quantitative Trait Loci (QTL) Mapping
Dong-Hai Xiong, Jian-Feng Liu, Yan-Fang Guo, Yan Guo, Tie-Lin Yang, Shu-Gui Chen, Hui Jiang, Yuan Chen, Fang Yang, Robert R. Recker and Hong-Wen Deng*

Imaging Techniques

17. In Situ Nuclear Organization of Regulator Machinery

Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein and Gary S. Stein*

18. Bioluminescent Imaging in Bone
Yoram Zilberman, Yossi Gafni, Gadi Pelled, Zulma Gazit, and Dan Gazit*

19. Micro Computed Tomography – A Method for the Nondestructive Evaluation of the Three-Dimensional Structure of Biological Specimens
Martin Stauber and Ralph Müller*

20. Fourier Transform Infrared (FT-IR) Microspectroscopy and Microscopic Imaging
Samuel Gourion-Arsiquaud, Paul A. West and Adele L. Boskey*


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