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Springer-Verlag New York, LLC
Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols / Edition 1

Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols / Edition 1

by Donald Armstrong


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Product Details

ISBN-13: 9781617372230
Publisher: Springer-Verlag New York, LLC
Publication date: 11/10/2010
Series: Methods in Molecular Biology , #196
Edition description: Softcover reprint of hardcover 1st ed. 2002
Pages: 356
Product dimensions: 5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Part I. Techniques for Histological Identification

Cyhemical Localization of H2O2 in Biological Tissues

E. Ann Ellis and Maria B. Grant

Localization of Intracellular Lipid Peroxides Using Tetramethylbenzidine Reaction for Transmission Electron Microscopy

E. Ann Ellis, Shigehiro Iwabuchi, Don Samuelson, and Donald Armstrong

The Immunohishemical Localization of Glutathione Peroxidase

Kiyoshi Akeo, Tadahisa Hiramitsu, and Keiichi Watanabe

4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody

Hideyuki J. Majima, Takako Nakanishi-Ueda, and Toshihiko Ozawa

Immunohishemical Detection of Protein Oxidation

Jürgen Frank, Alfonso Pompella, and Hans K. Biesalski

Indirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolated Cells

Alfonso Pompella, Silvia Dominici, Jürgen Frank, and Hans K. Biesalski

Part II. Techniques for Subcellular Localization

Methods for Studying the Binding of Advanced Glycated Proteins to Receptors for Advanced Glycation Endproducts

(AGE Receptors)

Paul J. Thornalley

Measurement of Blue Fluorescence as a Protein Marker for Oxidized Membranes

Kiyomi Kikugawa

X-Ray Diffraction Analysis of Membrane Structure Changes with Oxidative Stress

R. Preston Mason and Robert F. Jacob

Detection of Estrogen Receptor by In Situ Hybridization

Kaori Kobayashi and Hiroshi Kobayashi

Tumor Necrosis Factor-a Quantification and Expression by In Situ Hybridization

Tracey A. Ignatowski and Robert N. Spengler

Part III. Techniques for Molecular Biology

Translocation of p47phox and Activation of NADPH Oxidase in Mononuclear Cells

Ahmad Aljada, Husam Ghanim, and Paresh Dandona

Activation of Nuclear Factor-kB (NF-kB) in Mononuclear Cells (MNC)

Ahmad Aljada, Husam Ghanim, and Paresh Dandona

Assay of Intracellular Hydrogen Peroxide Generation in Activated Individual Neutrophils by Flow Cytometry

Yoshikazu Ito and David A. Lipschitz

DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs)

Gieri Camenisch, Roland H. Wenger, and Max Gassmann

Analysis of Heat-Shock Transcription Factor and Element-Binding Activity

Yong J. Lee

Slot-Blot Hybridization in Studying Gene Expression During Oxygen Deprivation

Enbo Ma and Gabriel G. Haddad

An Expression System for a Transporter of Iron and Other Metals

Michael D. Garrick and Kevin G. Dolan

Analysis of Gene Expression Following Oxidative Stress

Dana R. Crawford, Toshihide Suzuki, Jan Sesay, and Kelvin J. A. Davies

Measurement of Immunoglobulin G Oxidation by Western-Blot Analysis

Andrew Chow, Shahid Ahmed, Larrisa Chaplia, and Joseph Mattana

Thioredoxin and Redox Regulation of the Nuclear Receptor

Yuichi Makino, Kensaku Okamoto, and Hirotoshi Tanaka

Analysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNA

Xin Gen Lei and Wen-Hsing Cheng

Expression of Human Phospholipid Hydroperoxide Glutathione Peroxidase

Kunio Yagi, Sadaaki Komura, and Nobuko Ohishi

Part IV. Techniques for In Vivo/Ex Vivo/In Vitro Applications

Superoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence Method

Takashi Sakurai and Susumu Terakawa

In Vivo Detection of Transition Metals and Nitrosyl-Heme Complexes Using Ex Vivo Electron Paramagnetic Resonance Spectroscopy

David M. Hall and Garry R. Buettner

Spectral-Spatial Electron Paramagnetic Resonance Imaging (EPRI) in Skin Biopsies at 9.5 GHz

Jürgen Fuchs, Norbert Groth, and Thomas Herrling

In Vivo/In Situ Detection of Nitric Oxide Using Low-Frequency EPR Spectroscopy

Pei Tsai, Supatra Porasuphatana, Howard J. Halpern, Eugene D. Barth, and Gerald M. Rosen

Introduction of NOS II Gene into Primary Cultures of Bovine and Human Endothelial Cells

Guan-Liang Cao, Bin Zhang, Joseph B. Domachowske, and Gerald M. Rosen

Detection of Peroxynitrite-Induced Protein and DNA Modifications

Scott Lorch, Richard Lightfoot, Hiroshi Ohshima, László Virág, Qiping Chen, Caryn Hertkorn, Marie Weiss, Jose Souza, Harry Ischiropoulos, Vladimir Yermilov, Brigitte Pignatelli, Mituharu Masuda, and Csaba Szabó

Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

Pamela A. Szweda, Lin Tsai, and Luke I. Szweda

Liver Slice Technology as an In Vitro Model for Metabolic and Toxicity Studies

Sanjeev Thohan and Gerald M. Rosen

Cytofluorescence Techniques for the Visualization of Distinct Pools of Protein Thiols at the Single Cell Level

Alfonso Pompella, Silvia Dominici, Caterina Cambiaggi, Jürgen Frank, and Hans K. Biesalski

Preparation of Microspheres and Incorporation of Lipid Hydroperoxide for Sustained Release Studies

Donald Armstrong, Hideya Kimura, Kazushi Tamai, Tsutomu Yasukawa, Mohammed Afzal, and Richard W. Browne

Measurement of Carotenoids in the Living Primate Eye Using Resonance Raman Spectroscopy

Paul S. Bernstein and Werner Gellermann

Interstitial Photodynamic Therapy with Moving Exposure Fiber

Toru Hirano, Yasuo Hashimoto, Hideo Tanaka, Ichirou Yamada, and Kenji Hashimoto

Statistical Analysis: Receiver Operating Characteristic (ROC) Curve for Lipid Peroxidation

Enrique F. Schisterman


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