ISBN-10:
1617372811
ISBN-13:
9781617372810
Pub. Date:
11/10/2010
Publisher:
Springer-Verlag New York, LLC
PCR Cloning Protocols / Edition 2

PCR Cloning Protocols / Edition 2

by Bing-Yuan Chen, Harry W. Janes
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Product Details

ISBN-13: 9781617372810
Publisher: Springer-Verlag New York, LLC
Publication date: 11/10/2010
Series: Methods in Molecular Biology , #192
Edition description: Softcover reprint of hardcover 2nd ed. 2002
Pages: 439
Product dimensions: 6.10(w) x 9.25(h) x 0.35(d)

Table of Contents

Part I. Performing and Optimizing PCR

Polymerase Chain Reaction: Basic Principles and Routine Practice
Lori A. Kolmodin and David E. Birch

Computer Programs for PCR Primer Design and Analysis
Bing-Yuan Chen, Harry W. Janes, and Steve Chen

Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming
Kenneth H. Roux

XL PCR Amplification of Long Targets from Genomic DNA
Lori A. Kolmodin

Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments
Jyrki T. Aatsinki

Long Distance Reverse-Transcription PCR
Volker Thiel, Jens Herold, and Stuart G. Siddell

Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues
Abebe Akalu and Juergen K. V. Reichardt

GC-Rich Template Amplification by Inverse PCR: DNA Polymerase and Solvent Effects
Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez, and Jean Dusart

PCR Procedure for the Isolation of Trinucleotide Repeats
Teruaki Tozaki

Methylation-Specific PCR
Haruhiko Ohashi

Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads
Xin Huang, Zhenglong Yuan, and Xuetao Cao

Part II. Cloning PCR Products

Cloning PCR Products: An Overview
Baotai Guo and Yuping Bi

Using T4 DNA Polymerase to Generate Clonable PCR Products
Kai Wang

Enzyme-Free Cloning of PCR Products and Fusion Protein Expression
Brett A. Neilan and Daniel Tillett

Directional Restriction Site-Free Insertion of PCR Products into Vectors
Guo Jun Chen

Autosticky PCR: Directional Cloning of PCR Products with Preformed 5' Overhangs
József Gál and Miklós Kálmán

A Rapid and Simple Procedure for Direct Cloning of PCRProducts into Baculoviruses
Tamara S. Gritsun, Michael V. Mikhailov, and Ernest A. Gould

Part III. Mutagenesis and Recombination

PCR Approaches to DNA Mutagenesis and Recombination: An Overview
Binzhang Shen

In-Frame Cloning of Synthetic Genes Using PCR Inserts
James C. Pierce

Megaprimer PCR
Sailen Barik

PCR-Mediated Recombination: A General Method Applied to Construct Chimeric Infectious Molecular Clones
Guowei Fang, Barbara Weiser, Aloise Visosky, Timothy Moran, and Harold Burger

PCR Method for Generating Multiple Mutations at Adjacent Sites
Jiri Adamec

A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes
Franco Laccone

PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
Dario E. Lehoux and Roger C. Levesque

Staggered Extension Process (StEP) In Vitro Recombination
Anna Marie Aguinaldo and Frances Arnold

Random Mutagenesis by Whole-Plasmid PCR Amplification
Donghak Kim and F. Peter Guengerich

Part IV. Cloning Unknown Neighboring DNA

PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants: An Overview
Eric Ka-Wai Hui, Po-Ching Wang, and Szecheng J. Lo

Long Distance Vectorette PCR (LDV PCR)
James A. L. Fenton, Guy Pratt, and Gareth J. Morgan

Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA ('Cold-Start Method')
Michael Lardelli

Inverse PCR: cDNA Cloning
Sheng-He Huang

Inverse PCR: Genomic DNA Cloning
Ambrose Y. Jong, Anna T'ang, De-Pei Liu, and Sheng-He Huang

Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers
Sheng-He Huang, Hua-Yang Wu, and Ambrose Y. Jong

The Isolation of DNA Sequences Flanking Tn5 Trans

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