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PCR in Bioanalysis / Edition 1
     

PCR in Bioanalysis / Edition 1

by Stephen J Meltzer
 

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ISBN-10: 1617370525

ISBN-13: 9781617370526

Pub. Date: 11/10/2010

Publisher: Springer-Verlag New York, LLC

PCR in Bioanalysis offers powerful PCR-based prools and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses

Overview

PCR in Bioanalysis offers powerful PCR-based prools and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.

Product Details

ISBN-13:
9781617370526
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/10/2010
Series:
Methods in Molecular Biology Series , #92
Edition description:
Softcover reprint of hardcover 1st ed. 1998
Pages:
281
Product dimensions:
5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Application of Nucleic Acid Amplification in Clinical Microbiology

Gorm Lisby

Detection of Rodent Parvoviruses by PCR

David G. Besselsen

The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity

Darryl Shibata

Prenatal and Presymptomatic Diagnosis of Marfan Syndrome Using Fluorescence PCR and an Automated Sequencer

Mei Wang and Maurice Godfrey.

Measurement of TNF and iNOS mRNA Using cDNA-Equalized Reverse Transcriptase PCR

Jay K. Kolls and Jianming Xie

PCR Diagnosis of the Bovine Immunodeficiency-Like Virus

David L. Suarez and Cecelia A. Whetstone

PCR Analysis for the Identification of Porcine Reproductive and Respiratory Syndrome Virus in Boar Semen

Jane Christopher-Hennings and Eric A. Nelson

Quantitative and Discriminative Detection of Individual HIV-mRNA Subspecies by an RNase Mapping Assay

Catherine Mary, Hideo Akaoka, and Bernard Verrier

Detection of the Heat-Labile Toxin Coding Gene (LT-Gene) of Enterotoxigenic Escherichia coli

Séamus Fanning, Deirdre O'Meara, Lesley Cotter, John O'Mullane, and Bartley Cryan

Motif-Dependent Polymerase Chain Reaction (PCR): DNA Fingerprinting Entererotoxigenic Escherichia coli

Séamus Fanning, Deirdre O'Meara, Lesley Cotter, Paddy Greer, and Bartley Cryan

In Vitro Transcription/Translation Analysis for the Identification of Translation-Terminating Mutations

Michael C. Luce, Cameron G. Binnie, Lauren N. W. Kam-Morgan, and Matthew C. Cayouette

A Single-Tube Nested RT-PCR for the Detection of Ross River Virus

Loryn Sellner

Application of PCR to Transgenic Plants

Michael Wassenegger

Detection of Hepatitis C Virus RNA by Semiquantitative Reverse-Transcription PCR

Anderson S. Gaweco and David H. Van Thiel

Detection of Legionella Species in Bronchial Fluid by PCR

Gorm Lisby

Quantitative Measurement of mRNA Expression by Competitive RT-PCR

Joe O'Connell, Triona Goode, and Fergus Shanahan

PCR Detection of Toxoplasma gondii in Human Fetal Tissues

Tamás Tóth, István Sziller, and Zoltán Papp

Rapid Diagnosis of Pulmonary Tuberculosis Using Roche AMPLICORTM Mycobacterium tuberculosis PCR Test

Richard F. D'Amato and Albert Miller

The Use of Flow Cytometry and RT-PCR in the Detection of Circulating PSA-Positive Cells in Prostate Cancer

Emma J. Fadlon and Freddie C. Hamdy

Detection of Chlamydia trachomatis and Trichomonas vaginalis in the Vaginal Introitus, Posterior Vagina, and Endocervix by Polymerase Chain Reaction

Jan Jeremias, Vera Tolbert, and Steven S. Witkin

Detection and Isolation of Differentially Expressed Genes by PCR

John M. Abraham

Detection of Transgene Integrants and Homologous Recombinants in Mice by Polymerase Chain Reaction

Kristin M. Abraham, Nancy S. Longo, and Judith A. Hewitt

Direct Analysis for Familial Adenomatous Polyposis Mutations

Steven M. Powell

PCR Fingerprinting for Detection of Deleted or Amplified Sequences in Human Cancer

Takashi Kohno and Jun Yokota

Index

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