Plant Receptor Kinases: Methods and Protocols

Plant Receptor Kinases: Methods and Protocols

by Reidunn Birgitta Aalen (Editor)

Hardcover(1st ed. 2017)

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Product Details

ISBN-13: 9781493970629
Publisher: Springer New York
Publication date: 06/01/2017
Series: Methods in Molecular Biology , #1621
Edition description: 1st ed. 2017
Pages: 196
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

PART I Expression and Purification of PRKs


1. Expression of Plant Receptor Kinases in E. coli



Moneeza Akbar Agha, David Lightfoot, and Ahmed Jawaad Afzal



2. Expression of Plant Receptor Kinases in Yeast


María Laura Barberini and Jorge Muschietti



3. Expression of Plant Receptor Kinases in Tobacco BY-2 Cells


Hidefumi Shinohara and Yoshikatsu Matsubayashi



4. Cell-free Synthesis of Plant Receptor Kinases



Akira Nozawa, Keiichirou Nemoto, Shunsuke Nomura, Satoshi Yamanaka, Kohki Kido, and Tatsuya Sawasaki



5. Purification of Plant Receptor Kinases from Plant Plasma Membranes


Jin Suk Lee




PART II Ligand Binding and Activation of PRKs


6. Photoaffinity Labeling of Plant Receptor Kinases


Hidefumi Shinohara and Yoshikatsu Matsubayashi



7. Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases


Markus Albert and Ursula Fürst



PART III In silico Methods for Identification and Analyses of PRKs


8. Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis


Aleksia Vaattovaara, Jarkko Salojärvi, and Michael Wrzaczek



9. In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping


Russell J. S. Orr and Reidunn B. Aalen



PART IV Identification of Enzymatic Functions and Interacting Partners of PRKs


10. Co-immunoprecipitation of Plant Receptor Kinases



María Laura Barberini and Jorge Muschietti



11. Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell-free Systems


Keiichirou Nemoto, Akira Nozawa, Satoshi Yamanaka, Shunsuke Nomura, Kohki Kido, and Tatsuya Sawasaki



12. Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners


Matthew R Meyer, Shweta Shah, and A. Gururaj Rao



13. In vitro Assessment of Guanylyl Cyclase Activity of Plant Receptor Kinases


Misjudeen Raji and Chris Gehring



14. BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases


Cornelia Fischer, Margret Sauter, and Petra Dietrich



15. Pull Down Assay to Characterize Ca2+/Calmodulin Binding to Plant Receptor Kinases


Christine Kaufmann and Margret Sauter


PART V Imaging of PRK Dynamics



16. The use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases

Stefanie Weidtkamp-Peters and Yvonne Stahl



17. Detection and Analyses of Endocytosis of Plant Receptor Kinases



Jenna Loiseau and Silke Robatzek


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