Poly(ADP-Ribose) Polymerase: Methods and Protocols

Poly(ADP-Ribose) Polymerase: Methods and Protocols

by Alexei V. Tulin (Editor)

Paperback(Softcover reprint of the original 2nd ed. 2017)

$159.00
View All Available Formats & Editions
Choose Expedited Shipping at checkout for guaranteed delivery by Thursday, April 25

Product Details

ISBN-13: 9781493983612
Publisher: Springer New York
Publication date: 06/12/2018
Series: Methods in Molecular Biology , #1608
Edition description: Softcover reprint of the original 2nd ed. 2017
Pages: 528
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Part I: Detection and Quantification of pADPr



1. Quantitation of Poly(ADP-ribose) by Isotope Dilution Mass Spectrometry


Tabea Zubel, Rita Martello, Alexander Bürkle, and Aswin Mangerich



2. Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies


Eszter M. Horvath, Zsuzsanna K. Zsengellér, and Csaba Szabo



3. Detecting and Quantifying pADPr In Vivo



Yi-Chen Lai, Rajesh K. Aneja, Margaret A. Satchell, and Robert S.B. Clark



4. Compartment-Specific Poly-ADP-Ribose Formation as a Biosensor for Subcellular NAD Pools


Magali R. VanLinden, Marc Niere, Andrey A. Nikiforov, Mathias Ziegler, and Christian Dölle



5. Cell Cycle Resolved Measurements of Poly(ADP-ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry


Jone Michelena and Matthias Altmeyer



Part II: Identification of Protein Targets



6. Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe


Rory K. Morgan and Michael S. Cohen



7. ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method


Casey M. Daniels, Shao-En Ong, and Anthony K.L. Leung



8. Using Clickable NAD+ Analogues to Label Substrate Proteins of PARPs


Lu Zhang and Hening Lin



9. Identification of Protein Substrates of Specific PARP Enzymes Using Analog-Sensitive PARP Mutants and a ‘Clickable’ NAD+ Analog


Bryan A. Gibson and W. Lee Kraus



10. Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography – Tandem Mass Spectrometry


Mario Leutert, Vera Bilan, Peter Gehrig, and Michael O. Hottiger



11. Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography –Tandem Mass Spectrometry



Sara C. Larsen, Mario Leutert, Vera Bilan, Rita Martello, Stephanie Jungmichel, Clifford Young, Michael O. Hottiger, and Michael L. Nielsen



Part III: Functional Analysis



12. Poly(ADP-Ribose)-Dependent Chromatin Remodeling in DNA Repair


Théo Lebeaupin, Rebecca Smith, Sébastien Huet, and Gyula Timinszky



13. Methods to Assess the Role of Poly(ADP-Ribose) Polymerases in Regulating Mitochondrial Oxidative Function


Edit Mikó, Tünde Kovács, Tamás Fodor, and Péter Bai


14. Approaches for Investigating Translational Regulation Controlled by PARP1: Biotin-Based UV-Crosslinking and Luciferase Reporter Assay


Yingbiao Ji



15. Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1) – mRNA Targets by PAR-CLiP


Manana Mekishvilli, Elena Matveeva, and Yvonne Fondufe-Mittendorf



Part IV: Regulation of PARP-1 and Its Partners



16. Biochemical and Biophysical Methods for Analysis of Poly(ADP-Ribose) Polymerase 1 and Its Interactions with Chromatin


Maggie H. Chassé, Uma M. Muthurajan, Nicholas J. Clark, Michael A. Kramer, Srinivas Chakravarthy, Thomas Irving, and Karolin Luger



17. PARP-1 Interaction with and Activation by Histones and Nucleosomes


Colin Thomas, Elena Kotova, and Alexei V. Tulin



Part V: Designing and Testing PARP-1 Inhibitors



18. Strategies Employed for the Development of PARP Inhibitors



Stacie Canan, Karen Maegley, and Nicola J. Curtin



19. High-Throughput Colorimetric Assay for Identifying PARP-1 Inhibitors Using a Large Small-Molecule Collection


Elena Kotova and Alexei V. Tulin



20. Testing PARP Inhibitors Using a Subcutaneous Murine Xenograft Model


Peter Makhov, Sei Naito, and Vladimir M. Kolenko



21. In Vitro Long Term Proliferation Assays to Study Antiproliferative Effects of PARP Inhibitors on Cancer Cells


Heike Keilhack and Paul Chang



22. Use of Inosine Monophosphate Dehydrogenase Activity Assay to Determine the Specificity of PARP-1 Inhibitors


Sajitha Anthony, Jeffrey R. Peterson, and Yingbiao Ji



23. The Use of PARP Inhibitors in Cancer Therapy: Use as Adjuvant with Chemotherapy or Radiotherapy, Use as a Single Agent in Susceptible Patients, and Techniques Used to Identify Susceptible Patients



Sydney Shall, Terry Gaymes, Farzin Farzaneh, Nicola Curtin, and Ghulam J. Mufti



Part VI: Targeting the Understudied Components of pADPr Pathway



24. Purification of Recombinant Human PARP-3


Jean-Christophe Amé, Barbara Camuzeaux, Françoise Dantzer, and Valérie Schreiber



25. Purification of Recombinant Human PARG and Activity Assays


Jean-Christophe Amé, Éléa Héberlé, Barbara Camuzeaux, Françoise Dantzer, and Valérie Schreiber



26. Studying Catabolism of Protein ADP-Ribosylation


Luca Palazzo, Dominic I. James, Ian D. Waddell, and Ivan Ahel



27. Purification of DNA Damage-Dependent PARPs from E. coli for Structural and Biochemical Analysis


Marie-France Langelier, Jamin D. Steffen, Amanda A. Riccio, Michael McCauley, and John M. Pascal



28. Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach


Katie Pollock, Michael Ranes, Ian Collins, and Sebastian Guettler



29. Computational and Experimental Studies of ADP-Ribosylation


Robert G. Hammond, Xuan Tan, Matthew Chan, Anupam Goel, and Margaret A. Johnson

Customer Reviews

Most Helpful Customer Reviews

See All Customer Reviews