Poly(ADP-Ribose) Polymerase: Methods and Protocols

Poly(ADP-Ribose) Polymerase: Methods and Protocols

by Alexei V. Tulin (Editor)

Paperback(Softcover reprint of the original 2nd ed. 2017)

View All Available Formats & Editions
Choose Expedited Shipping at checkout for guaranteed delivery by Thursday, April 25

Product Details

ISBN-13: 9781493983612
Publisher: Springer New York
Publication date: 06/12/2018
Series: Methods in Molecular Biology , #1608
Edition description: Softcover reprint of the original 2nd ed. 2017
Pages: 528
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Part I: Detection and Quantification of pADPr

1. Quantitation of Poly(ADP-ribose) by Isotope Dilution Mass Spectrometry

Tabea Zubel, Rita Martello, Alexander Bürkle, and Aswin Mangerich

2. Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies

Eszter M. Horvath, Zsuzsanna K. Zsengellér, and Csaba Szabo

3. Detecting and Quantifying pADPr In Vivo

Yi-Chen Lai, Rajesh K. Aneja, Margaret A. Satchell, and Robert S.B. Clark

4. Compartment-Specific Poly-ADP-Ribose Formation as a Biosensor for Subcellular NAD Pools

Magali R. VanLinden, Marc Niere, Andrey A. Nikiforov, Mathias Ziegler, and Christian Dölle

5. Cell Cycle Resolved Measurements of Poly(ADP-ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry

Jone Michelena and Matthias Altmeyer

Part II: Identification of Protein Targets

6. Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe

Rory K. Morgan and Michael S. Cohen

7. ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method

Casey M. Daniels, Shao-En Ong, and Anthony K.L. Leung

8. Using Clickable NAD+ Analogues to Label Substrate Proteins of PARPs

Lu Zhang and Hening Lin

9. Identification of Protein Substrates of Specific PARP Enzymes Using Analog-Sensitive PARP Mutants and a ‘Clickable’ NAD+ Analog

Bryan A. Gibson and W. Lee Kraus

10. Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography – Tandem Mass Spectrometry

Mario Leutert, Vera Bilan, Peter Gehrig, and Michael O. Hottiger

11. Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography –Tandem Mass Spectrometry

Sara C. Larsen, Mario Leutert, Vera Bilan, Rita Martello, Stephanie Jungmichel, Clifford Young, Michael O. Hottiger, and Michael L. Nielsen

Part III: Functional Analysis

12. Poly(ADP-Ribose)-Dependent Chromatin Remodeling in DNA Repair

Théo Lebeaupin, Rebecca Smith, Sébastien Huet, and Gyula Timinszky

13. Methods to Assess the Role of Poly(ADP-Ribose) Polymerases in Regulating Mitochondrial Oxidative Function

Edit Mikó, Tünde Kovács, Tamás Fodor, and Péter Bai

14. Approaches for Investigating Translational Regulation Controlled by PARP1: Biotin-Based UV-Crosslinking and Luciferase Reporter Assay

Yingbiao Ji

15. Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1) – mRNA Targets by PAR-CLiP

Manana Mekishvilli, Elena Matveeva, and Yvonne Fondufe-Mittendorf

Part IV: Regulation of PARP-1 and Its Partners

16. Biochemical and Biophysical Methods for Analysis of Poly(ADP-Ribose) Polymerase 1 and Its Interactions with Chromatin

Maggie H. Chassé, Uma M. Muthurajan, Nicholas J. Clark, Michael A. Kramer, Srinivas Chakravarthy, Thomas Irving, and Karolin Luger

17. PARP-1 Interaction with and Activation by Histones and Nucleosomes

Colin Thomas, Elena Kotova, and Alexei V. Tulin

Part V: Designing and Testing PARP-1 Inhibitors

18. Strategies Employed for the Development of PARP Inhibitors

Stacie Canan, Karen Maegley, and Nicola J. Curtin

19. High-Throughput Colorimetric Assay for Identifying PARP-1 Inhibitors Using a Large Small-Molecule Collection

Elena Kotova and Alexei V. Tulin

20. Testing PARP Inhibitors Using a Subcutaneous Murine Xenograft Model

Peter Makhov, Sei Naito, and Vladimir M. Kolenko

21. In Vitro Long Term Proliferation Assays to Study Antiproliferative Effects of PARP Inhibitors on Cancer Cells

Heike Keilhack and Paul Chang

22. Use of Inosine Monophosphate Dehydrogenase Activity Assay to Determine the Specificity of PARP-1 Inhibitors

Sajitha Anthony, Jeffrey R. Peterson, and Yingbiao Ji

23. The Use of PARP Inhibitors in Cancer Therapy: Use as Adjuvant with Chemotherapy or Radiotherapy, Use as a Single Agent in Susceptible Patients, and Techniques Used to Identify Susceptible Patients

Sydney Shall, Terry Gaymes, Farzin Farzaneh, Nicola Curtin, and Ghulam J. Mufti

Part VI: Targeting the Understudied Components of pADPr Pathway

24. Purification of Recombinant Human PARP-3

Jean-Christophe Amé, Barbara Camuzeaux, Françoise Dantzer, and Valérie Schreiber

25. Purification of Recombinant Human PARG and Activity Assays

Jean-Christophe Amé, Éléa Héberlé, Barbara Camuzeaux, Françoise Dantzer, and Valérie Schreiber

26. Studying Catabolism of Protein ADP-Ribosylation

Luca Palazzo, Dominic I. James, Ian D. Waddell, and Ivan Ahel

27. Purification of DNA Damage-Dependent PARPs from E. coli for Structural and Biochemical Analysis

Marie-France Langelier, Jamin D. Steffen, Amanda A. Riccio, Michael McCauley, and John M. Pascal

28. Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach

Katie Pollock, Michael Ranes, Ian Collins, and Sebastian Guettler

29. Computational and Experimental Studies of ADP-Ribosylation

Robert G. Hammond, Xuan Tan, Matthew Chan, Anupam Goel, and Margaret A. Johnson

Customer Reviews

Most Helpful Customer Reviews

See All Customer Reviews