In this new, 5th edition of a highly popular text, undergraduate students are introduced to all the basic experimental techniques routinely used in practical biochemistry today. Most attention is given to techniques students will encounter in their practical classes, with the principles and theories behind them explained in detail to aid understanding. As a further aid to students, essential calculations and worked answers appear at the end of each chapter. 'Key terms to understand' are also included to help students thoroughly review each topic. No contemporary book on modern biochemical techniques would be complete without chapters on molecular biology, recombinant DNA technology, genetic analysis and biomolecular interactions, and these topics have been extensively covered in this new edition. The book is essential reading for all bioscience undergraduate students and pre-clinical medical students for whom practical biochemistry, molecular biology and immunology form part of the syllabus.
|Publisher:||Cambridge University Press|
|Edition description:||Older Edition|
|Product dimensions:||7.44(w) x 9.69(h) x 1.42(d)|
Table of ContentsPreface to the fifth edition; Abbreviations and SI units; 1. General principles of biochemical investigations I. Simpkins; 2. Molecular biology and basic techniques R. Rapley; 3. Recombinant DNA and genetic analysis R. Rapley; 4. Immunochemical techniques R. Thorpe and S. Thorpe; 5. Centrifugation techniques A. Griffiths; 6. Protein structure, purification and characterisation J. Walker; 7. Biomolecular interactions I. Enzymes K. Wilson; 8. Biomolecular interactions II. Cell surface receptors and transporters K. Wilson; 9. Spectroscopic techniques I. Atomic and molecular electronic spectroscopy D. B. Gordon; 10. Spectroscopic techniques II. Vibrational spectroscopy and electronic and nuclear spin orientation in magnetic fields D. B. Gordon; 11. Mass spectrometric techniques D. B. Gordon; 12. Electrophoretic techniques J. M. Walker; 13. Chromatographic techniques K. Wilson; 14. Radioisotope techniques R. J. Slater; 15. Electrochemical techniques P. K. Robinson.