Protein Crystallography: A Concise Guide

Protein Crystallography: A Concise Guide

by Eaton E. Lattman, Patrick J. Loll


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Product Details

ISBN-13: 9780801888069
Publisher: Johns Hopkins University Press
Publication date: 04/28/2008
Pages: 152
Product dimensions: 6.00(w) x 9.00(h) x 0.63(d)
Age Range: 18 Years

About the Author

Eaton E. Lattman is chair of the Department of Biophysics at the Johns Hopkins University and editor-in-chief of the journal Proteins: Structure, Function, and Genetics. Patrick J. Loll is the director of the Biochemistry Graduate Program at Drexel University College of Medicine.

Table of Contents

Preface     ix
Introduction     1
What Is X-ray Crystallography?     1
A Quick Look at Protein Crystals     12
Noncrystalline Specimens     15
Summary     17
Further Reading     17
A Physical Understanding of Diffraction     19
What Is Diffraction?     19
Diffraction from One-Dimensional Crystals     25
Reconstructing Images from Diffraction Patterns     29
Summary     35
Further Reading     36
Diffraction from Three-Dimensional Crystals     37
The Electron Density Function in Three Dimensions     37
Calculating the Diffraction Pattern from a Known Structure     49
Summary     53
Further Reading     54
Phase Determination by Isomorphous Replacement     55
Measuring the Phases     55
MAD Phasing     61
Fitting Models to Experimental Electron Density Maps     68
Summary     70
Further Reading     70
The Patterson Function     72
Definition of the Patterson Function     72
Using the Patterson Function to Locate Atoms     76
Summary     81
Further Reading     82
Phasing with Partially Known Structures     83
Difference Fourier Maps     83
Molecular Replacement     87
Summary     101
Further Reading     101
Crystallographic Refinement     102
Refinement Improves the Model     102
Least-Squares Refinement     103
Summary     111
Further Reading     112
Glossary     113
Index     131

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